Creatine supplementation attenuates pulmonary and systemic effects of lung ischemia and reperfusion injury

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Almeida, Francine Maria ^[1] ; Oliveira-Junior, Manoel Carneiro ^[2] ; Souza, Renato Aparecido ^{[2, 3]} ; Petroni, Ricardo Costa ^[4] ; Soto, Sonia Fatima ^[1] ; Soriano, Francisco Garcia ^[4] ; Camillo de Carvalho, Paulo Tarso ^[2] ; Albertini, Regiane ^[2] ; Damaceno-Rodrigues, Nilsa Regina ^[5] ; Quirino Santos Lopes, Fernanda Degobbi Tenorio ^[1] ; Castro-Faria-Neto, Hugo Caire ^[6] ; Martins, Milton Arruda ^[1] ; Dolhnikoff, Marisa ^[7] ; Pazetti, Rogerio ^[8] ; Vieira, Rodolfo Paula ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 20, Sao Paulo, SP - Brazil

^[2] Nove de Julho Univ UNINOVE, Lab Pulm & Exercise Immunol LABPEI, Sao Paulo, SP - Brazil

^[3] Inst Fed Educ Ciencia & Tecnol Sul Minas Gerais I, GEP CS, Campus Muzambinho, Muzambinho, MG - Brazil

^[4] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 51, Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 59, Sao Paulo, SP - Brazil

^[6] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Immunopharmacol, Rio De Janeiro, RJ - Brazil

^[7] Univ Sao Paulo, Sch Med, Dept Pathol LIM 05, Sao Paulo - Brazil

^[8] Univ Sao Paulo, Sch Med, Dept Cardiopneumol LIM 61, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF HEART AND LUNG TRANSPLANTATION; v. 35, n. 2, p. 242-250, FEB 2016.
• Citações Web of Science: 6

Resumo

BACKGROUND: Creatine (Cr) is a dietary supplement that presents beneficial effects in experimental models of heart and brain ischemia and reperfusion (I/R) injury. It can improve adenosine 5'-triphosphate generation and reduce cell damage This study evaluated the effects of Cr supplementation in a model of lung PR. METHODS: Forty male Wistar rats were divided into 4 groups: sham operated, Cr+sham, I/R, and Cr+I/R. We investigated the effects of 5 days of Cr supplementation (0.5 g/kg/day by gavage) before left pulmonary artery ischemia (90 minutes) and reperfusion (120 minutes) on pulmonary and systemic response. RESULTS: Cr inhibited the I/R-induced increase in exhaled nitric oxide (p < 0.05), total cells (p < 0.01), and neutrophils (p < 0.001) in bronchoalveolar lavage fluid and in the systemic circulation (p < 0.001). The levels of interleulcin-1 beta (p < 0.05), tissue damping, and tissue elastance (p < 0.05) were also minimized Cr also inhibited pulmonary edema formation (total proteins in bronchoalveolar lavage fluid, p < 0.001; histologic edema index, p < 0.001) and neutrophils accumulation in lung tissue (p < 0.001). As possible mechanisms underlying Cr effects, we observed a reduced expression of caspase 3 (p < 0.05), reduced expression of Toll-like receptor (TLR) 4, and increased expression of TLR7 in lung tissue (p < 0.001). CONCLUSIONS: Cr supplementation presents pulmonary and systemic protective effects in acute lung injury induced by I/R in rats. These beneficial effects seem to be related to the anti-inflammatory and antioxidant properties of Cr and modulation of TLRs. (C) 2016 International Society for Heart and Lung Transplantation. All rights reserved. (AU)

• Processo FAPESP: 12/15165-2 - Papel da sinalização purinérgica e da sinalização SOCS-JAK-STAT nos efeitos antiinflamatórios do treinamento aeróbio em modelos experimentais de asma e em indivíduos asmáticos
• Beneficiário: Rodolfo de Paula Vieira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 14/14604-8 - Papel da sinalização purinérgica nos efeitos anti-inflamatórios do treinamento aeróbio em um modelo de asma induzida por ácaro de poeira doméstica
• Beneficiário: Manoel Carneiro de Oliveira Junior
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/14848-1 - Efeitos da suplementação com creatina na lesão de isquemia e reperfusão após transplante pulmonar unilateral em ratos
• Beneficiário: Rogerio Pazetti
• Modalidade de apoio: Auxílio à Pesquisa - Regular