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| Autor(es): Mostrar menos - |
Almeida, Francine Maria
[1]
;
Oliveira-Junior, Manoel Carneiro
[2]
;
Souza, Renato Aparecido
[2, 3]
;
Petroni, Ricardo Costa
[4]
;
Soto, Sonia Fatima
[1]
;
Soriano, Francisco Garcia
[4]
;
Camillo de Carvalho, Paulo Tarso
[2]
;
Albertini, Regiane
[2]
;
Damaceno-Rodrigues, Nilsa Regina
[5]
;
Quirino Santos Lopes, Fernanda Degobbi Tenorio
[1]
;
Castro-Faria-Neto, Hugo Caire
[6]
;
Martins, Milton Arruda
[1]
;
Dolhnikoff, Marisa
[7]
;
Pazetti, Rogerio
[8]
;
Vieira, Rodolfo Paula
[2]
Número total de Autores: 15
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 20, Sao Paulo, SP - Brazil
[2] Nove de Julho Univ UNINOVE, Lab Pulm & Exercise Immunol LABPEI, Sao Paulo, SP - Brazil
[3] Inst Fed Educ Ciencia & Tecnol Sul Minas Gerais I, GEP CS, Campus Muzambinho, Muzambinho, MG - Brazil
[4] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 51, Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 59, Sao Paulo, SP - Brazil
[6] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Immunopharmacol, Rio De Janeiro, RJ - Brazil
[7] Univ Sao Paulo, Sch Med, Dept Pathol LIM 05, Sao Paulo - Brazil
[8] Univ Sao Paulo, Sch Med, Dept Cardiopneumol LIM 61, Sao Paulo - Brazil
Número total de Afiliações: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF HEART AND LUNG TRANSPLANTATION; v. 35, n. 2, p. 242-250, FEB 2016. |
| Citações Web of Science: | 6 |
| Resumo | |
BACKGROUND: Creatine (Cr) is a dietary supplement that presents beneficial effects in experimental models of heart and brain ischemia and reperfusion (I/R) injury. It can improve adenosine 5'-triphosphate generation and reduce cell damage This study evaluated the effects of Cr supplementation in a model of lung PR. METHODS: Forty male Wistar rats were divided into 4 groups: sham operated, Cr+sham, I/R, and Cr+I/R. We investigated the effects of 5 days of Cr supplementation (0.5 g/kg/day by gavage) before left pulmonary artery ischemia (90 minutes) and reperfusion (120 minutes) on pulmonary and systemic response. RESULTS: Cr inhibited the I/R-induced increase in exhaled nitric oxide (p < 0.05), total cells (p < 0.01), and neutrophils (p < 0.001) in bronchoalveolar lavage fluid and in the systemic circulation (p < 0.001). The levels of interleulcin-1 beta (p < 0.05), tissue damping, and tissue elastance (p < 0.05) were also minimized Cr also inhibited pulmonary edema formation (total proteins in bronchoalveolar lavage fluid, p < 0.001; histologic edema index, p < 0.001) and neutrophils accumulation in lung tissue (p < 0.001). As possible mechanisms underlying Cr effects, we observed a reduced expression of caspase 3 (p < 0.05), reduced expression of Toll-like receptor (TLR) 4, and increased expression of TLR7 in lung tissue (p < 0.001). CONCLUSIONS: Cr supplementation presents pulmonary and systemic protective effects in acute lung injury induced by I/R in rats. These beneficial effects seem to be related to the anti-inflammatory and antioxidant properties of Cr and modulation of TLRs. (C) 2016 International Society for Heart and Lung Transplantation. All rights reserved. (AU) | |
| Processo FAPESP: | 12/15165-2 - Papel da sinalização purinérgica e da sinalização SOCS-JAK-STAT nos efeitos antiinflamatórios do treinamento aeróbio em modelos experimentais de asma e em indivíduos asmáticos |
| Beneficiário: | Rodolfo de Paula Vieira |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 14/14604-8 - Papel da sinalização purinérgica nos efeitos anti-inflamatórios do treinamento aeróbio em um modelo de asma induzida por ácaro de poeira doméstica |
| Beneficiário: | Manoel Carneiro de Oliveira Junior |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 13/14848-1 - Efeitos da suplementação com creatina na lesão de isquemia e reperfusão após transplante pulmonar unilateral em ratos |
| Beneficiário: | Rogerio Pazetti |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |