Creatine supplementation attenuates pulmonary and systemic effects of lung ischemia and reperfusion injury

Author(s):Show less -

Almeida, Francine Maria ^[1] ; Oliveira-Junior, Manoel Carneiro ^[2] ; Souza, Renato Aparecido ^{[2, 3]} ; Petroni, Ricardo Costa ^[4] ; Soto, Sonia Fatima ^[1] ; Soriano, Francisco Garcia ^[4] ; Camillo de Carvalho, Paulo Tarso ^[2] ; Albertini, Regiane ^[2] ; Damaceno-Rodrigues, Nilsa Regina ^[5] ; Quirino Santos Lopes, Fernanda Degobbi Tenorio ^[1] ; Castro-Faria-Neto, Hugo Caire ^[6] ; Martins, Milton Arruda ^[1] ; Dolhnikoff, Marisa ^[7] ; Pazetti, Rogerio ^[8] ; Vieira, Rodolfo Paula ^[2]

Affiliation

^[1] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 20, Sao Paulo, SP - Brazil

^[2] Nove de Julho Univ UNINOVE, Lab Pulm & Exercise Immunol LABPEI, Sao Paulo, SP - Brazil

^[3] Inst Fed Educ Ciencia & Tecnol Sul Minas Gerais I, GEP CS, Campus Muzambinho, Muzambinho, MG - Brazil

^[4] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 51, Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 59, Sao Paulo, SP - Brazil

^[6] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Immunopharmacol, Rio De Janeiro, RJ - Brazil

^[7] Univ Sao Paulo, Sch Med, Dept Pathol LIM 05, Sao Paulo - Brazil

^[8] Univ Sao Paulo, Sch Med, Dept Cardiopneumol LIM 61, Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF HEART AND LUNG TRANSPLANTATION; v. 35, n. 2, p. 242-250, FEB 2016.
• Web of Science Citations: 6

Abstract

BACKGROUND: Creatine (Cr) is a dietary supplement that presents beneficial effects in experimental models of heart and brain ischemia and reperfusion (I/R) injury. It can improve adenosine 5'-triphosphate generation and reduce cell damage This study evaluated the effects of Cr supplementation in a model of lung PR. METHODS: Forty male Wistar rats were divided into 4 groups: sham operated, Cr+sham, I/R, and Cr+I/R. We investigated the effects of 5 days of Cr supplementation (0.5 g/kg/day by gavage) before left pulmonary artery ischemia (90 minutes) and reperfusion (120 minutes) on pulmonary and systemic response. RESULTS: Cr inhibited the I/R-induced increase in exhaled nitric oxide (p < 0.05), total cells (p < 0.01), and neutrophils (p < 0.001) in bronchoalveolar lavage fluid and in the systemic circulation (p < 0.001). The levels of interleulcin-1 beta (p < 0.05), tissue damping, and tissue elastance (p < 0.05) were also minimized Cr also inhibited pulmonary edema formation (total proteins in bronchoalveolar lavage fluid, p < 0.001; histologic edema index, p < 0.001) and neutrophils accumulation in lung tissue (p < 0.001). As possible mechanisms underlying Cr effects, we observed a reduced expression of caspase 3 (p < 0.05), reduced expression of Toll-like receptor (TLR) 4, and increased expression of TLR7 in lung tissue (p < 0.001). CONCLUSIONS: Cr supplementation presents pulmonary and systemic protective effects in acute lung injury induced by I/R in rats. These beneficial effects seem to be related to the anti-inflammatory and antioxidant properties of Cr and modulation of TLRs. (C) 2016 International Society for Heart and Lung Transplantation. All rights reserved. (AU)

• FAPESP's process: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals
• Grantee: Rodolfo de Paula Vieira
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 14/14604-8 - Role of purinergic signaling in the antiinflammatory effects of aerobic training in a model of asthma induced by house dust mite
• Grantee: Manoel Carneiro de Oliveira Junior
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/14848-1 - Effects of creatine supplementation in ischemia-reperfusion injury after unilateral lung transplantation in rats
• Grantee: Rogerio Pazetti
• Support Opportunities: Regular Research Grants