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Role of purinergic signaling in the antiinflammatory effects of aerobic training in a model of asthma induced by house dust mite

Grant number: 14/14604-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): December 01, 2014
Effective date (End): March 31, 2017
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Rodolfo de Paula Vieira
Grantee:Manoel Carneiro de Oliveira Junior
Home Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
Associated research grant:12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals, AP.JP

Abstract

Asthma is a chronic airway inflammatory disease that results in impairment of quality of life of asthmatics. In the last years, several studies have demonstrated that aerobic physical training (AT) performed properly results in anti-inflammatory effects to the airways, either in experimental models of asthma or to asthmatic individuals. Thus, the present project aims to evaluate the effects of AT in experimental models of asthma using house dust mite (HDM) (dermatophagoides pteronyssinus). To this purpose, in the experimental model of chronic asthma (HDM 100ug/mouse; 1x/week, 7 weeks), the animals will receive the HDM by intra-tracheal via during the first 3 weeks and then the AT will begin after the third week (for evaluation of the therapeutic effect of AT). The AT will be performed in a treadmill for mice, during 4 weeks, 1h/session, 5x/week. Twenty-four hours after the last session of training and HDM administration, the adenosine triphosphate (ATP) levels will be measured in the bronchoalveolar lavage (BAL), the number of cells (total and differential) in BAL and in blood, the number of eosinophils, macrophages and lymphocytes in the airways wall, the levels of pro-inflammatory mediators (Th2 cytokines - IL-4, IL-5 and IL-13 and also Th1 cytokine - TNF-alpha) and the levels of anti-inflammatory cytokines (IL-10, IL-1ra) in BAL and serum, the expression of sTNFR1 and sTNFR2 in the lungs, the expression of purinergic receptors (P2X7, P2Y2 and P2Y6) in the lungs and in the lymphatic organs (spleen, lymphnodes and bone marrow), as well as the airways remodeling and the bronchial hyperresponsiveness. In addition, the levels of IL-4, IL-5, IL-13, IL-10 and IL-1ra in the supernatant of cell culture of total cells from spleen, lymph nodes and bone marrow re-stimulated in vitro with HDM will be measured. (AU)

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Scientific publications (14)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE CAMARGO, ANDERSON ALVES; SILVA DE CASTRO, REJANE AGNELO; VIEIRA, RODOLFO P.; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; DE ARAUJO, AMANDA APARECIDA; DE ANGELIS, KATIA; RACHED, SAMIA ZAHI; ATHANAZIO, RODRIGO ABENSUR; STELMACH, RAFAEL; DAL CORSO, SIMONE. Systemic Inflammation and Oxidative Stress in Adults with Bronchiectasis: Association with Clinical and Functional Features. Clinics, v. 76, 2021. Web of Science Citations: 0.
EL-MAFARJEH, ELIAS; MARTINS, GISELE HENRIQUE CARDOSO; PROBST, JESSICA JORGE; SANTOS-DIAS, ALANA; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; DE BARROS, MARCELO PAES; DE OLIVEIRA, LUIS VICENTE FRANCO; DE ANDRADE, LEANDRO DAMAS; DA PALMA, RENATA KELLY; MORAES-FERREIRA, RENILSON; DE CAMARGO HIZUME-KUNZLER, DEBORAH; BACHI, ANDRE LUIS LACERDA; VIEIRA, RODOLFO P. Exercise Improves Lung Inflammation, but Not Lung Remodeling and Mechanics in a Model of Bleomycin-Induced Lung Fibrosis. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2020, OCT 17 2020. Web of Science Citations: 0.
DAVINO-CHIOVATTO, JAIME EDUARDO; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; MACKENZIE, BREANNE; SANTOS-DIAS, ALANA; ALMEIDA-OLIVEIRA, ANA ROBERTA; JONCO AQUINO-JUNIOR, JEFFERSON COMIN; BRITO, AURILEIA APARECIDA; RIGONATO-OLIVEIRA, NICOLE CRISTINE; DAMACENO-RODRIGUES, NILSA REGINA; LIGEIRO OLIVEIRA, ANA PAULA; SILVA, ALESSANDRO PEREIRA; CONSOLIM-COLOMBO, FERNANDA MARCIANO; AIMBIRE, FLAVIO; CASTRO-FARIA-NETO, HUGO CAIRE; VIEIRA, RODOLFO PAULA. Montelukast, Leukotriene Inhibitor, Reduces LPS-Induced Acute Lung Inflammation and Human Neutrophil Activation. ARCHIVOS DE BRONCONEUMOLOGIA, v. 55, n. 11, p. 573-580, NOV 2019. Web of Science Citations: 1.
GARCIA, MONIQUE; SANTOS-DIAS, ALANA; LACERDA BACHI, ANDRE LUIS; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; ANDRADE-SOUZA, ADILSON SANTOS; FERREIRA, SERGIO CESAR; JONCO AQUINO-JUNIOR, JEFFERSON COMIN; ALMEIDA, FRANCINE MARIA; RIGONATO-OLIVEIRA, NICOLE CRISTINE; LIGEIRO OLIVEIRA, ANA PAULA; BAGGIO SAVIO, LUIZ EDUARDO; COUTINHO-SILVA, ROBSON; MUELLER, TOBIAS; IDZKO, MARCO; SIEPMANN, TIMO; VIEIRA, RODOLFO PAULA. Creatine supplementation impairs airway inflammation in an experimental model of asthma involving P2 x 7 receptor. European Journal of Immunology, v. 49, n. 6, p. 928-939, JUN 2019. Web of Science Citations: 1.
SIERRA, ANA PAULA; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; ALMEIDA, FRANCINE MARIA; BENETTI, MARINO; OLIVEIRA, RODRIGO; FELIX, SORAIA NOGUEIRA; GENARO, ISABELLA SANTOS; SARAIVA ROMANHOLO, BEATRIZ MANGUEIRA; GHORAYEB, NABIL; PEDUTI DAL MOLIN KISS, MARIA AUGUSTA; CURY-BOAVENTURA, MARIA FERNANDA; PESQUERO, JOAO BOSCO; VIEIRA, RODOLFO PAULA. Impairment on Cardiopulmonary Function after Marathon: Role of Exhaled Nitric Oxide. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2019. Web of Science Citations: 1.
JONCO AQUINO-JUNIOR, JEFFERSON COMIN; MACKENZIE, BREANNE; ALMEIDA-OLIVEIRA, ANA ROBERTA; MARTINS, ANA CAROLINA; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; BRITTO, AURILEA APARECIDA; ARANTES-COSTA, FERNANDA MAGALHAES; DAMACENO-RODRIGUES, NILSA REGINA; CALDINI, ELIA GARCIA; LIGEIRO DE OLIVEIRA, ANA PAULA; GUADAGNINI, DIOZE; LEIRIA, LUIZ OSORIO; RICARDO, DJALMA RABELO; ABDALLA SAAD, MARIO JOSE; VIEIRA, RODOLFO PAULA. Aerobic exercise inhibits obesity-induced respiratory phenotype. CYTOKINE, v. 104, p. 46-52, APR 2018. Web of Science Citations: 1.
SILVA-RENNO, ADRIANO; BALDIVIA, GUILHERME CRISITANINI; OLIVEIRA-JUNIOR, MANOEL C.; RODRIGUES BRANDAO-RANGEL, MAYSA ALVES; EL-MAFARJEH, ELIAS; DOLHNIKOFF, MARISA; MAUAD, THAIS; BRITTO, JOSE MARA; NASCIMENTO SALDIVA, PAULO HILARIO; FRANCO OLIVEIRA, LUIS VICENTE; LIGEIRO-OLIVEIRA, ANA P.; GRAUDENZ, GUSTAVO SILVEIRA; VIEIRA, RODOLFO PAULA. Exercise Performed Concomitantly with Particulate Matter Exposure Inhibits Lung Injury. INTERNATIONAL JOURNAL OF SPORTS MEDICINE, v. 39, n. 2, p. 133-140, FEB 2018. Web of Science Citations: 2.
DURIGON, THOMAS STRAVINSKAS; MACKENZIE, BREANNE; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; SANTOS-DIAS, ALANA; DE ANGELIS, KATIA; MALFITANO, CHRISTIANO; DA PALMA, RENATA KELLY; GUERRA, JULIANA MORENO; DAMACENO-RODRIGUES, NILSA REGINA; CALDINI, ELIA GARCIA; DE ALMEIDA, FRANCINE MARIA; AQUINO-SANTOS, HELIDA CRISTINA; RIGONATO-OLIVEIRA, NICOLE CRISTINE; LEAL DE OLIVEIRA, DANIELLE BRUNA; AIMBIRE, FLAVIO; LIGEIRO DE OLIVEIRA, ANA PAULA; FRANCO DE OLIVEIRA, LUIZ VICENTE; DURIGON, EDISON LUIZ; HIEMSTRA, PIETER S.; VIEIRA, RODOLFO P. Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice. JOURNAL OF INNATE IMMUNITY, v. 10, n. 4, p. 279-290, 2018. Web of Science Citations: 2.
MORAES, GABRIEL DA CUNHA; VITORETTI, LUANA BEATRIZ; DE BRITO, AURILEIA APARECIDA; ALVES, CINTIA ESTEFANO; RIGONATO DE OLIVEIRA, NICOLE CRISTINE; DIAS, ALANA DOS SANTOS; TELES MATOS, YVES SILVA; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; FRANCO OLIVEIRA, LUIS VICENTE; DA PALMA, RENATA KELLY; CANDEO, LARISSA CARBONERA; LINO-DOS-SANTOS-FRANCO, ADRIANA; RATTO TEMPESTINE HORLIANA, ANNA CAROLINA; GIMENES, JOAO ANTONIO JUNIOR; AIMBIRE, FLAVIO; VIEIRA, RODOLFO PAULA; LIGEIRO-DE-OLIVEIRA, ANA PAULA. Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2018. Web of Science Citations: 2.
RODRIGUES BRANDAO-RANGEL, MAYSA ALVES; LACERDA BACHI, ANDRE LUIS; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; ABBASI, ASGHAR; SILVA-RENNO, ADRIANO; DE BRITO, AURILEIA APARECIDA; LIGEIRO DE OLIVEIRA, ANA PAULA; TOLEDO-ARRUDA, ALESSANDRA CHOQUETA; BELVISI, MARIA GABRIELA; VIEIRA, RODOLFO PAULA. Exercise Inhibits the Effects of Smoke-Induced COPD Involving Modulation of STAT3. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2017. Web of Science Citations: 4.
ANDRADE-SOUSA, ADILSON SANTOS; PEREIRA, PAULO ROGERIO; MACKENZIE, BREANNE; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; ASSUMPCAO-NETO, ERASMO; RODRIGUES BRANDAO-RANGEL, MAYSA ALVES; DAMACENO-RODRIGUES, NILSA REGINA; CALDINI, ELIA GARCIA; PEREIRA VELOSA, ANA PAULA; TEODORO, WALCY ROSOLIA; LIGEIRO DE OLIVEIRA, ANA PAULA; DOLHNIKOFF, MARISA; EICKELBERG, OLIVER; VIEIRA, RODOLFO PAULA. Aerobic Exercise Attenuated Bleomycin-Induced Lung Fibrosis in Th2-Dominant Mice. PLoS One, v. 11, n. 9 SEP 27 2016. Web of Science Citations: 5.
PEREIRA, PAULO ROGERIO; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; MACKENZIE, BREANNE; DAVINO CHIOVATTO, JAIME EDUARDO; MATOS, YVES; GREIFFO, FLAVIA REGINA; RIGONATO-OLIVEIRA, NICOLE CRISTINE; BRUGEMMAN, THAYSE REGINA; DELLE, HUMBERTO; IDZKO, MARCO; ALBERTINI, REGIANE; LIGEIRO OLIVEIRA, ANA PAULA; DAMACENO-RODRIGUES, NILSA REGINA; CALDINI, ELIA GARCIA; FERNANDEZ, ISIS ENSIL; CASTRO-FARIA-NETO, HUGO CAIRE; DOLHNIKOFF, MARISA; EICKELBERG, OLIVER; VIEIRA, RODOLFO PAULA. Exercise Reduces Lung Fibrosis Involving Serotonin/Akt Signaling. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, v. 48, n. 7, p. 1276-1284, JUL 2016. Web of Science Citations: 11.
ALBERCA-CUSTODIO, RICARDO WESLEY; GREIFFO, FLAVIA REGINA; MACKENZIE, BREANNE; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; ANDRADE-SOUSA, ADILSON SANTOS; GRAUDENZ, GUSTAVO SILVEIRA; GOMES SANTOS, ANGELA BATISTA; DAMACENO-RODRIGUES, NILSA REGINA; CASTRO-FARIA-NETO, HUGO CAIRE; ARANTES-COSTA, FERNANDA MAGALHAES; MARTINS, MILTON DE ARRUDA; ABBASI, ASGHAR; LIN, CHIN JIA; IDZKO, MARCO; LIGEIRO OLIVEIRA, ANA PAULA; NORTHOFF, HINNAK; VIEIRA, RODOLFO PAULA. Aerobic Exercise Reduces Asthma Phenotype by Modulation of the Leukotriene Pathway. FRONTIERS IN IMMUNOLOGY, v. 7, JUN 14 2016. Web of Science Citations: 9.
ALMEIDA, FRANCINE MARIA; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; SOUZA, RENATO APARECIDO; PETRONI, RICARDO COSTA; SOTO, SONIA FATIMA; SORIANO, FRANCISCO GARCIA; CAMILLO DE CARVALHO, PAULO TARSO; ALBERTINI, REGIANE; DAMACENO-RODRIGUES, NILSA REGINA; QUIRINO SANTOS LOPES, FERNANDA DEGOBBI TENORIO; CASTRO-FARIA-NETO, HUGO CAIRE; MARTINS, MILTON ARRUDA; DOLHNIKOFF, MARISA; PAZETTI, ROGERIO; VIEIRA, RODOLFO PAULA. Creatine supplementation attenuates pulmonary and systemic effects of lung ischemia and reperfusion injury. JOURNAL OF HEART AND LUNG TRANSPLANTATION, v. 35, n. 2, p. 242-250, FEB 2016. Web of Science Citations: 6.

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