| Full text | |
| Author(s): Show less - |
Almeida, Francine Maria
[1]
;
Oliveira-Junior, Manoel Carneiro
[2]
;
Souza, Renato Aparecido
[2, 3]
;
Petroni, Ricardo Costa
[4]
;
Soto, Sonia Fatima
[1]
;
Soriano, Francisco Garcia
[4]
;
Camillo de Carvalho, Paulo Tarso
[2]
;
Albertini, Regiane
[2]
;
Damaceno-Rodrigues, Nilsa Regina
[5]
;
Quirino Santos Lopes, Fernanda Degobbi Tenorio
[1]
;
Castro-Faria-Neto, Hugo Caire
[6]
;
Martins, Milton Arruda
[1]
;
Dolhnikoff, Marisa
[7]
;
Pazetti, Rogerio
[8]
;
Vieira, Rodolfo Paula
[2]
Total Authors: 15
|
| Affiliation: | [1] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 20, Sao Paulo, SP - Brazil
[2] Nove de Julho Univ UNINOVE, Lab Pulm & Exercise Immunol LABPEI, Sao Paulo, SP - Brazil
[3] Inst Fed Educ Ciencia & Tecnol Sul Minas Gerais I, GEP CS, Campus Muzambinho, Muzambinho, MG - Brazil
[4] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 51, Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 59, Sao Paulo, SP - Brazil
[6] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Immunopharmacol, Rio De Janeiro, RJ - Brazil
[7] Univ Sao Paulo, Sch Med, Dept Pathol LIM 05, Sao Paulo - Brazil
[8] Univ Sao Paulo, Sch Med, Dept Cardiopneumol LIM 61, Sao Paulo - Brazil
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | JOURNAL OF HEART AND LUNG TRANSPLANTATION; v. 35, n. 2, p. 242-250, FEB 2016. |
| Web of Science Citations: | 6 |
| Abstract | |
BACKGROUND: Creatine (Cr) is a dietary supplement that presents beneficial effects in experimental models of heart and brain ischemia and reperfusion (I/R) injury. It can improve adenosine 5'-triphosphate generation and reduce cell damage This study evaluated the effects of Cr supplementation in a model of lung PR. METHODS: Forty male Wistar rats were divided into 4 groups: sham operated, Cr+sham, I/R, and Cr+I/R. We investigated the effects of 5 days of Cr supplementation (0.5 g/kg/day by gavage) before left pulmonary artery ischemia (90 minutes) and reperfusion (120 minutes) on pulmonary and systemic response. RESULTS: Cr inhibited the I/R-induced increase in exhaled nitric oxide (p < 0.05), total cells (p < 0.01), and neutrophils (p < 0.001) in bronchoalveolar lavage fluid and in the systemic circulation (p < 0.001). The levels of interleulcin-1 beta (p < 0.05), tissue damping, and tissue elastance (p < 0.05) were also minimized Cr also inhibited pulmonary edema formation (total proteins in bronchoalveolar lavage fluid, p < 0.001; histologic edema index, p < 0.001) and neutrophils accumulation in lung tissue (p < 0.001). As possible mechanisms underlying Cr effects, we observed a reduced expression of caspase 3 (p < 0.05), reduced expression of Toll-like receptor (TLR) 4, and increased expression of TLR7 in lung tissue (p < 0.001). CONCLUSIONS: Cr supplementation presents pulmonary and systemic protective effects in acute lung injury induced by I/R in rats. These beneficial effects seem to be related to the anti-inflammatory and antioxidant properties of Cr and modulation of TLRs. (C) 2016 International Society for Heart and Lung Transplantation. All rights reserved. (AU) | |
| FAPESP's process: | 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals |
| Grantee: | Rodolfo de Paula Vieira |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 14/14604-8 - Role of purinergic signaling in the antiinflammatory effects of aerobic training in a model of asthma induced by house dust mite |
| Grantee: | Manoel Carneiro de Oliveira Junior |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 13/14848-1 - Effects of creatine supplementation in ischemia-reperfusion injury after unilateral lung transplantation in rats |
| Grantee: | Rogerio Pazetti |
| Support Opportunities: | Regular Research Grants |