Role of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic exercise in an experimental model of asthma

• Grant number: 14/07500-1
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): July 01, 2014
• Effective date (End): October 31, 2015
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Rodolfo de Paula Vieira
• Grantee: Ana Roberta Almeida Comin
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
• Associated research grant: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals, AP.JP

Abstract

Asthma is a chronic airway inflammatory disease that results in impairment of quality of life. In the last years, several studies have demonstrated that Aerobic Training (AT) performed properly results in anti-inflammatory effects to the airways, either in experimental models of asthma and to asthmatic individuals. However, although few cellular effects have been investigated (still only in animals' models), the possible extra and intracellular mediators underlying the effects of AT on asthma never were investigated, except to NF-kB. Thus, the present project aims to evaluate the effects of AT on SOCS, JAK and STAT proteins signaling on the lungs as possible modulators of the anti-inflammatory effects of aerobic training in an experimental model of asthma (by using House Dust Mite - HDM). For this purpose, the animals will receive intra-tracheal administration of HDM (100ug) on days 0, 7, 14, 21, 28, 35 e 42 and will be submitted to AT (for evaluation of therapeutic effect) begining on day 17 during 5 weeks. Twenty-four hours after the last session of training and allergen challenge, the animals will be assessed to the inflammatory profile in the bronchoalveolar lavage and in the lung tissue, to the levels of pro-inflammatory cytokines (IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-12, IL-13, IL-17, IL-23) and to the levels of anti-inflammatory cytokines (IL-10, IL-1ra) and also for the expression of SOCS1, SOCS2, SOCS3, JAK2, STAT3, STAT5 e STAT6 in the lungs, as well as for the airway remodeling, and bronchial hyperresponsiveness. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALMEIDA-OLIVEIRA, A. R.; AQUINO, JR., J. C. J.; ABBASI, A.; SANTOS-DIAS, A.; OLIVEIRA, JR., M. C.; ALBERCA-CUSTODIO, R. W.; RIGONATO-OLIVEIRA, N. C.; SALLEY-DIAS, L. P.; DAMACENO-RODRIGUES, N. R.; CALDINI, E. G.; et al. Effects of Aerobic Exercise on Molecular Aspects of Asthma: Involvement of SOCS-JAK-STAT. EXERCISE IMMUNOLOGY REVIEW, v. 25, p. 98-110, 2019. (12/16498-5, 12/15165-2, 14/12755-9, 14/07500-1, 12/21519-1)

DAVINO-CHIOVATTO, JAIME EDUARDO; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; MACKENZIE, BREANNE; SANTOS-DIAS, ALANA; ALMEIDA-OLIVEIRA, ANA ROBERTA; JONCO AQUINO-JUNIOR, JEFFERSON COMIN; BRITO, AURILEIA APARECIDA; RIGONATO-OLIVEIRA, NICOLE CRISTINE; DAMACENO-RODRIGUES, NILSA REGINA; LIGEIRO OLIVEIRA, ANA PAULA; et al. Montelukast, Leukotriene Inhibitor, Reduces LPS-Induced Acute Lung Inflammation and Human Neutrophil Activation. ARCHIVOS DE BRONCONEUMOLOGIA, v. 55, n. 11, p. 573-580, NOV 2019. (12/15165-2, 14/23196-0, 14/14604-8, 14/07500-1, 14/12755-9)

JONCO AQUINO-JUNIOR, JEFFERSON COMIN; MACKENZIE, BREANNE; ALMEIDA-OLIVEIRA, ANA ROBERTA; MARTINS, ANA CAROLINA; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; BRITTO, AURILEA APARECIDA; ARANTES-COSTA, FERNANDA MAGALHAES; DAMACENO-RODRIGUES, NILSA REGINA; CALDINI, ELIA GARCIA; LIGEIRO DE OLIVEIRA, ANA PAULA; et al. Aerobic exercise inhibits obesity-induced respiratory phenotype. CYTOKINE, v. 104, p. 46-52, APR 2018. (14/14604-8, 14/07500-1, 12/15165-2)