An in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrep/Cobas TaqMan HBV tests for the quantification of hepatitis B virus DNA

Autor(es)

de Torres Santos, Ana Paula ^[1] ; Levi, Jose Eduardo ^[2] ; Lemos, Marcilio Figueiredo ^[3] ; Calux, Samira Julien ^[3] ; Oba, Isabel Takano ^[3] ; Moreira, Regina Celia ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Hosp Clin, Div Lab Cent, Lab Imunol, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Trop Med, Virol Lab, Sao Paulo, SP - Brazil

^[3] Adolfo Lutz Inst, Ctr Virol, Nucleo Doencas Sanguineas & Sexuais, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 111, n. 2, p. 134-140, FEB 2016.
• Citações Web of Science: 0

Resumo

This study aimed to standardise an in-house real-time polymerase chain reaction (rtPCR) to allow quantification of hepatitis B virus (HBV) DNA in serum or plasma samples, and to compare this method with two commercial assays, the Cobas Amplicor HBV monitor and the Cobas AmpliPrep/Cobas TaqMan HBV test. Samples from 397 patients from the state of São Paulo were analysed by all three methods. Fifty-two samples were from patients who were human immunodeficiency virus and hepatitis C virus positive, but HBV negative. Genotypes were characterised, and the viral load was measure in each sample. The in-house rtPCR showed an excellent success rate compared with commercial tests; inter-assay and intra-assay coefficients correlated with commercial tests (r = 0.96 and r = 0.913, p < 0.001) and the in-house test showed no genotype-dependent differences in detection and quantification rates. The in-house assay tested in this study could be used for screening and quantifying HBV DNA in order to monitor patients during therapy. (AU)

• Processo FAPESP: 09/53086-4 - Padronização e implantação da técnica de PCR em tempo real para o diagnóstico da Hepatite B
• Beneficiário: Regina Célia Moreira
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa em Políticas Públicas para o SUS