Plasma Steroid Dynamics in Late- and Near-term Naturally and Artificially Conceived Bovine Pregnancies as Elucidated by Multihormone High-resolution LC-MS/MS

Autor(es)

Martins-Junior, Helio A. ^{[1, 2]} ; Pinaffi, Fabio L. V. ^[3] ; Simas, Rosineide C. ^{[1, 4]} ; Tarouco, Adriana K. ^{[5, 6]} ; Ferreira, Christina R. ^[1] ; Silva, Luciano A. ^[3] ; Nogueira, Guilherme P. ^[4] ; Meirelles, Flavio V. ^[5] ; Eberlin, Marcos N. ^[1] ; Perecin, Felipe ^[5]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, Campinas, SP - Brazil

^[2] AB SCIEX Brazil, BR-04719002 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Lab Theriogenol Dr OJ Ginther, Dept Vet Med, Sch Anim Sci & Food Engn FZEA, BR-13635900 Pirassununga, SP - Brazil

^[4] Sao Paulo State Univ, Dept Prod & Anim Hlth, Sch Vet Med, BR-16050680 Aracatuba, SP - Brazil

^[5] Univ Sao Paulo, Lab Mol Morphophysiol & Dev, Dept Vet Med, Sch Anim Sci & Food Engn, BR-13635900 Pirassununga, SP - Brazil

^[6] State Fdn Agr Res, Res Ctr Iwar Beckman, BR-96400970 Hulha Negra, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Endocrinology; v. 155, n. 12, p. 5011-5023, DEC 2014.
• Citações Web of Science: 2

Resumo

The plasma levels of corticosteroids and sex steroids during pregnancy are key indicators of mammalian placental function and the onset of parturition. Steroid hormones are believed to be disturbed in pregnancies produced using assisted reproductive technologies (ARTs) due to placental dysfunction and the frequently observed lack of parturition signals. To elucidate the plasma steroid dynamics, a liquid chromatography-tandem mass spectrometry method was developed and used to determine the levels of corticosteroids (corticosterone, 11-deoxycortisol, and cortisol) and their direct precursors (progesterone and 17 alpha-OH-progesterone) as well as sex steroids (androstenedione, estrone, estrone sulfate, testosterone, and 17 beta-estradiol) in bovine plasma. The levels of these 10 steroids in recipient cows carrying naturally conceived (control), in vitro fertilized (IVF), or cloned (somatic cell nuclear transfer) conceptuses were compared during late-term pregnancy (30 days before parturition), during near-term pregnancy (1 day before parturition), and on the day of parturition (day 0). Significant differences were observed among the corticosteroid levels: higher levels of corticosterone, 11-deoxycortisol, and cortisol were detected in cloned pregnancies at day 30; lower levels of corticosterone were observed in ART-derived pregnancies at days 1 and 0; and estrone and estradiol levels were higher in IVF pregnancies through out the final development. These results suggested an upregulation of the P450(C11) and P450(C21) enzymes 30 days before parturition in somatic cell nuclear transfer pregnancies and an over-activation of the aromatase enzyme in IVF pregnancies. Taken together, the monitoring of multiple steroid hormones revealed that the pregnancies obtained using ART exhibited plasma steroid concentration dynamics compatible with the dysregulation of steroidogenic tissues. (AU)

• Processo FAPESP: 10/05806-5 - Prenhez de clones bovinos e os enigmas da síndrome da pré-eclâmpsia: desenvolvimento de um modelo experimental para estudos de distúrbios vasculares no útero e na placenta
• Beneficiário: Luciano Andrade Silva
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 07/52003-2 - Produção comercial de clones bovinos
• Beneficiário: Tiago Henrique Camara de Bem
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE
• Processo FAPESP: 10/15167-0 - Desenvolvimento e Validação Analítica de Método de Quantificação Simultânea de Estradiol e Progesterona por LC-MS/MS em Soro/Plasma Sanguíneo Bovino
• Beneficiário: Rosineide Costa Simas
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/51677-2 - Técnicas modernas em espectrometria de massas e desenvolvimento de novas aplicações em ciências: química, bioquímica, materiais, forense, medicina, alimentos, farmácia e veterinária
• Beneficiário: Marcos Nogueira Eberlin
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 07/58786-9 - Produção comercial de clones bovinos
• Beneficiário: Felipe Perecin
• Modalidade de apoio: Bolsas no Brasil - Pesquisa Inovativa em Pequenas Empresas - PIPE