Autor(es)

Fratini, Paula ^[1] ; Oliveira Carreira, Ana Claudia ^{[2, 3]} ; Alcantara, Dayane ^[1] ; de Oliveira e Silva, Fernanda Menezes ^[1] ; Rodrigues, Marcio Nogueira ^[1] ; Miglino, Maria Angelica ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, BR-05508270 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, NUCEL Cell & Mol Therapy Ctr, BR-05508270 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Clin Med, NETCEM Ctr Studies Cell & Mol Therapy, BR-05508270 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Research in Veterinary Science; v. 104, p. 71-76, FEB 2016.
• Citações Web of Science: 7

Resumo

Yolk sac (YS) is the site of blood-cell production where primitive erythroid cells originate and complete their maturation. YS is a source of precursor cells, however its differentiation potential and suitability for cell therapies are not well described. YS can be a cell source when neovascularization is required. This study characterized YS canine cells, transduced with VEGF, to analyze then using Immunocytochemistry, flow cytometry and real time PCR. Immunocytochemistry: positive expression for CD105, PCNA, VEGF and vWF, flow cytometry for CD105, VEGF, PCNA, OCT-4 and RT-qPCR for VEGF, CD31, CD105, PCNA and FLT-1, indicating that these cells have characteristics of endothelial progenitor and pluripotency. After transduction, the YS cells changed their morphology and showed endothelial-like cells. We suggest, because of their cell surface phenotype as well as their capacity to differentiate into endothelial-like cells, that canine YS represents a source of cells for neovascularization therapies. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/01335-8 - Caracterização e diferenciação de linhagens celulares vitelinas hematopoiéticas do cão comparadas com células de medula óssea para futura aplicação na medicina veterinária regenerativa
• Beneficiário: Paula Fratini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado