| Full text | |
| Author(s): |
Fratini, Paula
[1]
;
Oliveira Carreira, Ana Claudia
[2, 3]
;
Alcantara, Dayane
[1]
;
de Oliveira e Silva, Fernanda Menezes
[1]
;
Rodrigues, Marcio Nogueira
[1]
;
Miglino, Maria Angelica
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, BR-05508270 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, NUCEL Cell & Mol Therapy Ctr, BR-05508270 Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Clin Med, NETCEM Ctr Studies Cell & Mol Therapy, BR-05508270 Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Research in Veterinary Science; v. 104, p. 71-76, FEB 2016. |
| Web of Science Citations: | 7 |
| Abstract | |
Yolk sac (YS) is the site of blood-cell production where primitive erythroid cells originate and complete their maturation. YS is a source of precursor cells, however its differentiation potential and suitability for cell therapies are not well described. YS can be a cell source when neovascularization is required. This study characterized YS canine cells, transduced with VEGF, to analyze then using Immunocytochemistry, flow cytometry and real time PCR. Immunocytochemistry: positive expression for CD105, PCNA, VEGF and vWF, flow cytometry for CD105, VEGF, PCNA, OCT-4 and RT-qPCR for VEGF, CD31, CD105, PCNA and FLT-1, indicating that these cells have characteristics of endothelial progenitor and pluripotency. After transduction, the YS cells changed their morphology and showed endothelial-like cells. We suggest, because of their cell surface phenotype as well as their capacity to differentiate into endothelial-like cells, that canine YS represents a source of cells for neovascularization therapies. (C) 2015 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 10/01335-8 - Caracterização e diferenciação de linhagens celulares vitelinas hematopoiéticas do cão comparadas com células de medula óssea para futura aplicação na medicina veterinária regenerativa |
| Grantee: | Paula Fratini |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |