Study of the polymorphisms of cyclooxygenase-2 (-765G > C) and 5-lipoxygenase ( 1708G > A) in patients with colorectal cancer

Autor(es)

Marques Pimenta, Celia Aparecida ^[1] ; Moreira Latini, Flavia Roche ^[1] ; De Lima, Jacqueline Miranda ^[1] ; Da Silva, Tiago Donizetti ^[1] ; Felipe, Aledson Vitor ^[1] ; De Lima Pazine, Vanessa Maria ^[1] ; Forones, Nora Manoukian ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Div Gastroenterol, BR-04023900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Oncology Letters; v. 7, n. 2, p. 513-518, FEB 2014.
• Citações Web of Science: 1

Resumo

Colorectal cancer (CRC) is the fourth most common cause of cancer-related mortality worldwide. Genetic alterations have been associated with an increased risk of cancer and greater tumor aggressiveness. Cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) genes are important in cell cycle regulation, tumor growth and prostaglandin synthesis. The aim of the present study was to investigate the association between polymorphisms in the COX-2 and 5-LOX genes and the risk of CRC. A case-control study was conducted in patients with CRC matched for gender and age to a control group. DNA was extracted from peripheral leukocytes, and the polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism and gene sequencing. A specific questionnaire was applied to evaluate smoking, excessive alcohol consumption, physical activity, non-steroidal anti-inflammatory drug use and meat, fiber and fat intake. A total of 185 patients with CRC and 146 controls were studied. The heterozygous GC genotype of the COX-2 gene polymorphism was the most common in the two groups (60.0% in CRC patients and 52.7% in controls). The CC genotype was associated with an increased risk of CRC (odds ratio, 3.63; 95% confidence interval, 1.31-10.1; P=0.013). The homozygous wild-type genotype of the 5-LOX gene polymorphism was detected in 72.4% of the CRC patients and in 71.2% of the control subjects. The homozygous mutant genotype (CC) of the COX-2 gene is an independent risk factor for CRC. No association was found between 5-LOX genotypes and CRC. (AU)

• Processo FAPESP: 09/14388-5 - Polimorfismo da COX-2 (-765 G>C) e da 5-LOX (G-1708A) em pacientes com câncer colorretal
• Beneficiário: Nora Manoukian Forones
• Modalidade de apoio: Auxílio à Pesquisa - Regular