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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Study of the polymorphisms of cyclooxygenase-2 (-765G > C) and 5-lipoxygenase ( 1708G > A) in patients with colorectal cancer

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Author(s):
Marques Pimenta, Celia Aparecida [1] ; Moreira Latini, Flavia Roche [1] ; De Lima, Jacqueline Miranda [1] ; Da Silva, Tiago Donizetti [1] ; Felipe, Aledson Vitor [1] ; De Lima Pazine, Vanessa Maria [1] ; Forones, Nora Manoukian [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo, Div Gastroenterol, BR-04023900 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Oncology Letters; v. 7, n. 2, p. 513-518, FEB 2014.
Web of Science Citations: 1
Abstract

Colorectal cancer (CRC) is the fourth most common cause of cancer-related mortality worldwide. Genetic alterations have been associated with an increased risk of cancer and greater tumor aggressiveness. Cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) genes are important in cell cycle regulation, tumor growth and prostaglandin synthesis. The aim of the present study was to investigate the association between polymorphisms in the COX-2 and 5-LOX genes and the risk of CRC. A case-control study was conducted in patients with CRC matched for gender and age to a control group. DNA was extracted from peripheral leukocytes, and the polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism and gene sequencing. A specific questionnaire was applied to evaluate smoking, excessive alcohol consumption, physical activity, non-steroidal anti-inflammatory drug use and meat, fiber and fat intake. A total of 185 patients with CRC and 146 controls were studied. The heterozygous GC genotype of the COX-2 gene polymorphism was the most common in the two groups (60.0% in CRC patients and 52.7% in controls). The CC genotype was associated with an increased risk of CRC (odds ratio, 3.63; 95% confidence interval, 1.31-10.1; P=0.013). The homozygous wild-type genotype of the 5-LOX gene polymorphism was detected in 72.4% of the CRC patients and in 71.2% of the control subjects. The homozygous mutant genotype (CC) of the COX-2 gene is an independent risk factor for CRC. No association was found between 5-LOX genotypes and CRC. (AU)

FAPESP's process: 09/14388-5 - Polymorphism of COX-2 (-765 G>C) and 5-LOX G-1708A) in colorectal cancer patients
Grantee:Nora Manoukian Forones
Support Opportunities: Regular Research Grants