RP-HPLC method for estimation of sesamin in two Zanthoxylum species

Autor(es)

Henrique, Camila Y. ^[1] ; Bertanha, Camila S. ^[1] ; Alvarenga, Tavane A. ^[1] ; Silva, Marcio L. A. ^[1] ; Cunha, Wilson R. ^[1] ; Januario, Ana H. ^[1] ; Pauletti, Patricia M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, Nucleo Pesquisa Ciencias Exatase & Tecnol, Ave Dr Armando Salles de Oliveira 201, BR-14404600 Franca, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Liquid Chromatography & Related Technologies; v. 39, n. 2, p. 65-69, JAN 20 2016.
• Citações Web of Science: 1

Resumo

Zanthoxylum naranjillo and Z. tingoassuiba (Rutaceae) are traditional herbal medicines with various biological activities including anti-inflammatory, analgesic, and antimalarial action. In this work, we have developed a simple HPLC-DAD method to quantify sesamin, a bioactive lignan present in Z. naranjillo and Z. tingoassuiba; egonol was the internal standard. According to the developed method, 11.07 +/- 1.66, 8.69 +/- 0.95, and 15.11 +/- 0.72 mu g/mL sesamin was present in the ethanol extract of Z. naranjillo leaves, in the methanol extract of Z. naranjillo leaves, and in the methanol extract of Z. tingoassuiba bark, respectively. The limits of detection and quantification were 0.32 and 1.06 mu g/mL, respectively. The developed method can be easily applied during routine analysis of sesamin in these medicinal plants. (AU)

• Processo FAPESP: 08/01268-9 - Estudo fitoquímico e avaliação das atividades antimalárica, antimicrobiana e de inibição da enzima acetilcolinesterase de espécies nativas do cerrado do estado de São Paulo
• Beneficiário: Ana Helena Januário
• Modalidade de apoio: Auxílio à Pesquisa - Regular