Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão)

Autor(es)

Alessandra Cury Machado ^[1] ; Patrícia Fernandes Sartori ^[2] ; Carla Andreotti Damante ^[3] ; Anne Lígia Dokkedal ^[4] ; Rodrigo Cardoso de Oliveira ^[5]

Afiliação do(s) autor(es)

^[1] USP. Faculdade de Odontologia de Bauru - Brasil

^[2] USP. Faculdade de Odontologia de Bauru - Brasil

^[3] USP. Faculdade de Odontologia de Bauru - Brasil

^[4] USP. Faculdade de Odontologia de Bauru - Brasil

^[5] USP. Faculdade de Odontologia de Bauru - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Archives of Biology and Technology; v. 59, 2016-03-08.

Resumo

ABSTRACT The aim of this study was to evaluate the effect of ethanolic "aroeira" (Myracrodruon urundeuva) extract on the viability of human gingival fibroblast. For this, fibroblasts (2x103 cells/well) were plated in a 96-well plate and incubated for 24 h; the medium (Eagle's medium modified by Dulbecco - DMEM) supplemented with 10% fetal bovine serum was replaced by DMEM with different ethanolic extract concentration (0, 0.1, 1, 10, 100, and 1000μg / mL). The fibroblast viability was analyzed after 48,72, and 96 h by the neutral red capture test and violet crystal. The "aroeira" extract, at high concentrations (100 and 1000 µg/mL) caused decrease in both cellular viability tests (p<0.05). However, dilutions between 0.1 and 10 µg/mL did not affect the viability of the cells. It was concluded that "aroeira" extract was able to change the gingival fibroblast viability, and this effect was concentration dependent. (AU)

• Processo FAPESP: 10/02026-9 - Avaliação da influência do extrato de aroeira na adesão e proliferação por fibroblatos humanos e expressão de MMP-2 e 9 in vitro
• Beneficiário: Rodrigo Cardoso de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/22243-7 - Efeito do extrato de aroeira na adesão e proliferação de fibroblastos
• Beneficiário: Patricia Fernandes Sartori
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica