| Full text | |
| Author(s): |
Alessandra Cury Machado
[1]
;
Patrícia Fernandes Sartori
[2]
;
Carla Andreotti Damante
[3]
;
Anne Lígia Dokkedal
[4]
;
Rodrigo Cardoso de Oliveira
[5]
Total Authors: 5
|
| Affiliation: | [1] USP. Faculdade de Odontologia de Bauru - Brasil
[2] USP. Faculdade de Odontologia de Bauru - Brasil
[3] USP. Faculdade de Odontologia de Bauru - Brasil
[4] USP. Faculdade de Odontologia de Bauru - Brasil
[5] USP. Faculdade de Odontologia de Bauru - Brasil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Brazilian Archives of Biology and Technology; v. 59, 2016-03-08. |
| Abstract | |
ABSTRACT The aim of this study was to evaluate the effect of ethanolic "aroeira" (Myracrodruon urundeuva) extract on the viability of human gingival fibroblast. For this, fibroblasts (2x103 cells/well) were plated in a 96-well plate and incubated for 24 h; the medium (Eagle's medium modified by Dulbecco - DMEM) supplemented with 10% fetal bovine serum was replaced by DMEM with different ethanolic extract concentration (0, 0.1, 1, 10, 100, and 1000μg / mL). The fibroblast viability was analyzed after 48,72, and 96 h by the neutral red capture test and violet crystal. The "aroeira" extract, at high concentrations (100 and 1000 µg/mL) caused decrease in both cellular viability tests (p<0.05). However, dilutions between 0.1 and 10 µg/mL did not affect the viability of the cells. It was concluded that "aroeira" extract was able to change the gingival fibroblast viability, and this effect was concentration dependent. (AU) | |
| FAPESP's process: | 10/02026-9 - Evaluations of aroeira"s extract influence in adhesion and proliferate of human fibroblast and MMP-2 and MMP-9 expression in vitro |
| Grantee: | Rodrigo Cardoso de Oliveira |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/22243-7 - Effect of aroeira extract on adherence and proliferation of fibroblasts |
| Grantee: | Patricia Fernandes Sartori |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |