Impact of Doxorubicin Treatment on the Physiological Functions of White Adipose Tissue

Autor(es):Mostrar menos -

Biondo, Luana Amorim ^[1] ; Lima Junior, Edson Alves ^[1] ; Souza, Camila Oliveira ^[1] ; Cruz, Maysa Mariana ^[2] ; Cunha, Roberta D. C. ^[2] ; Alonso-Vale, Maria Isabel ^[2] ; Oyama, Lila Missae ^[3] ; Oller Nascimento, Claudia M. ^[3] ; Pimentel, Gustavo Duarte ^[4] ; dos Santos, Ronaldo V. T. ^[5] ; Lira, Fabio Santos ^[6] ; Rosa Neto, Jose Cesar ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Cellular Biol & Dev, Immunometab Res Grp, Sao Paulo, SP - Brazil

^[2] Fed Univ Sao Paulo UNIFESP, Inst Environm Sci Chem & Pharmaceut, Dept Biol Sci, Sao Paulo, SP - Brazil

^[3] Fed Univ Sao Paulo UNIFESP, Dept Physiol, Physiol Nutr Discipline, Sao Paulo, SP - Brazil

^[4] Univ Fed Goias, Fac Nursing & Nutr, Goiania, Go - Brazil

^[5] Univ Fed Sao Paulo UNIFESP, Dept Psychobiol, Sao Paulo, SP - Brazil

^[6] State Univ Sao Paulo Julio de Mesquita Filho UNES, Dept Phys Educ, Presidente Prudente, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 11, n. 3 MAR 25 2016.
• Citações Web of Science: 12

Resumo

White adipose tissue (WAT) plays a fundamental role in maintaining energy balance and important endocrine functions. The loss of WAT modifies adipokine secretion and disrupts homeostasis, potentially leading to severe metabolic effects and a reduced quality of life. Doxorubicin is a chemotherapeutic agent used clinically because of its good effectiveness against various types of cancer. However, doxorubicin has deleterious effects in many healthy tissues, including WAT, liver, and skeletal and cardiac muscles. Our objective was to investigate the effects of doxorubicin on white adipocytes through in vivo and in vitro experiments. Doxorubicin reduced the uptake of glucose by retroperitoneal adipocytes and 3T3-L1 cells via the inhibition of AMP-activated protein kinase Thr172 phosphorylation and glucose transporter 4 content. Doxorubicin also reduced the serum level of adiponectin and, to a greater extent, the expression of genes encoding lipogenic (Fas and Acc) and adipogenic factors (Pparg, C/ebpa, and Srebp1c) in retroperitoneal adipose tissue. In addition, doxorubicin inhibited both lipogenesis and lipolysis and reduced the hormone-sensitive lipase and adipose tissue triacylglycerol lipase protein levels. Therefore, our results demonstrate the impact of doxorubicin on WAT. These results are important to understand some side effects observed in patients receiving chemotherapy and should encourage new adjuvant treatments that aim to inhibit these side effects. (AU)

• Processo FAPESP: 13/09367-4 - Efeitos metabólicos da doxorrubicina no músculo esquelético e tecido adiposo: possível papel adjuvante do treinamento físico e metformina
• Beneficiário: José Cesar Rosa Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular