In vitro evaluation of adhesion/proliferation of human gingival fibroblasts on demineralized root surfaces by toluidine blue O in antimicrobial photodynamic therapy

Autor(es)

Damante, Carla Andreotti ^[1] ; Ducati, Paula ^[1] ; Ferreira, Rafael ^[1] ; Salmeron, Samira ^[1] ; Ragghianti Zangrando, Mariana Schutzer ^[1] ; Rubo de Rezende, Maria Lucia ^[1] ; Passanezi Sant'Ana, Adriana Campos ^[1] ; Aguiar Greghi, Sebastido Luiz ^[1] ; Magalhaes, Ana Carolina ^[2]

Afiliação do(s) autor(es)

^[1] Bauru Sch Dent, Discipline Periodont, Sao Paulo - Brazil

^[2] Bauru Sch Dent, Discipline Biochem, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 13, p. 303-307, MAR 2016.
• Citações Web of Science: 4

Resumo

Background: Antimicrobial photodynamic therapy (aPDT) in Dentistry has important effects as bacterial destruction in areas with periodontal disease. Some dyes applied in aPDT could present low pH and, consequently, result in tooth demineralization. This study evaluated demineralization produced by aPDT with toluidine blue 0 (TBO) at low pH and analyzed adhesion/proliferation of human gingival fibroblasts (HGF). Methods: In the 1st phase, bovine enamel and root dentin fragments received 2 treatments: PDT4 group (TBO-100 mu g/ml-pH 4-60 s) plus laser (660 nm, 45 J/cm(2), 1.08J, 30 mW, 30 s, spot 0.024 cm(2), 1.25 W/cm(2), sweeping, non-contact) and CA group (citric acid plus tetracycline-pH 1-180 s). Surface hardness loss and tooth wear were statistically analyzed (Student's t test, ANOVA/Tukey, p<0.05). In the 2nd phase, human dentin fragments were divided in C (control group-scaling and root planing), PDT4 and CA. HGF (10(4), 5th passage) were cultured on these fragments for 24, 48 and 72 h and counted in scanning electron microscopy photographs. Number of HGF was analyzed using repeated-measures ANOVA and Turkey (p < 0.05). Results: Percentage of surface hardness loss was similar in dentin for PDT4 (71.5%) and CA(76.1%) (p > 0.05) and higher in enamel for CA (68.0%) compared to PDT4 (34.1%) (p < 0.05). In respect to wear, no difference was found between PDT4 (dentin: 12.58 mu m, enamel: 12.19 mu m respectively) and CA (dentin: 11.74 mu m and enamel: 11.03 mu m) (p > 0.05). Number of HGF was higher after 72 h in CA group (2.66, p<0.05) compared to PDT4 (2.2) and C (1.33). Conclusion: PDT4 is not as aggressive as CA for enamel. However, dentin demineralized promoted by PDT4 does not stimulate HGF adhesion and proliferation as CA. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/06738-9 - Avaliação de materiais, instrumentos e protocolos de irrigação empregados no tratamento endodôntico, por meio da microscopia eletrônica de varredura e micro análise de raios X.
• Beneficiário: Marco Antonio Hungaro Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/13328-9 - Efeito da terapia fotodinâmica na desmineralização superficial do esmalte e da dentina radicular bovinos in vitro
• Beneficiário: Paula Ducati
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica