In vitro evaluation of adhesion/proliferation of human gingival fibroblasts on demineralized root surfaces by toluidine blue O in antimicrobial photodynamic therapy

Author(s)

Damante, Carla Andreotti ^[1] ; Ducati, Paula ^[1] ; Ferreira, Rafael ^[1] ; Salmeron, Samira ^[1] ; Ragghianti Zangrando, Mariana Schutzer ^[1] ; Rubo de Rezende, Maria Lucia ^[1] ; Passanezi Sant'Ana, Adriana Campos ^[1] ; Aguiar Greghi, Sebastido Luiz ^[1] ; Magalhaes, Ana Carolina ^[2]

Affiliation

^[1] Bauru Sch Dent, Discipline Periodont, Sao Paulo - Brazil

^[2] Bauru Sch Dent, Discipline Biochem, Sao Paulo - Brazil

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 13, p. 303-307, MAR 2016.
• Web of Science Citations: 4

Abstract

Background: Antimicrobial photodynamic therapy (aPDT) in Dentistry has important effects as bacterial destruction in areas with periodontal disease. Some dyes applied in aPDT could present low pH and, consequently, result in tooth demineralization. This study evaluated demineralization produced by aPDT with toluidine blue 0 (TBO) at low pH and analyzed adhesion/proliferation of human gingival fibroblasts (HGF). Methods: In the 1st phase, bovine enamel and root dentin fragments received 2 treatments: PDT4 group (TBO-100 mu g/ml-pH 4-60 s) plus laser (660 nm, 45 J/cm(2), 1.08J, 30 mW, 30 s, spot 0.024 cm(2), 1.25 W/cm(2), sweeping, non-contact) and CA group (citric acid plus tetracycline-pH 1-180 s). Surface hardness loss and tooth wear were statistically analyzed (Student's t test, ANOVA/Tukey, p<0.05). In the 2nd phase, human dentin fragments were divided in C (control group-scaling and root planing), PDT4 and CA. HGF (10(4), 5th passage) were cultured on these fragments for 24, 48 and 72 h and counted in scanning electron microscopy photographs. Number of HGF was analyzed using repeated-measures ANOVA and Turkey (p < 0.05). Results: Percentage of surface hardness loss was similar in dentin for PDT4 (71.5%) and CA(76.1%) (p > 0.05) and higher in enamel for CA (68.0%) compared to PDT4 (34.1%) (p < 0.05). In respect to wear, no difference was found between PDT4 (dentin: 12.58 mu m, enamel: 12.19 mu m respectively) and CA (dentin: 11.74 mu m and enamel: 11.03 mu m) (p > 0.05). Number of HGF was higher after 72 h in CA group (2.66, p<0.05) compared to PDT4 (2.2) and C (1.33). Conclusion: PDT4 is not as aggressive as CA for enamel. However, dentin demineralized promoted by PDT4 does not stimulate HGF adhesion and proliferation as CA. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 12/06738-9 - Evaluation of materials, instruments and protocols of irrigation used in Endodontic treatment, by means of scanning electron microscopy and micro analysis of x-ray.
• Grantee: Marco Antonio Hungaro Duarte
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/13328-9 - Effect of photodynamic therapy on superficial demineralization of bovine enamel and root dentin in vitro
• Grantee: Paula Ducati
• Support Opportunities: Scholarships in Brazil - Scientific Initiation