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Influence of antimicrobial photodynamic therapy on carious lesion: clinical analysis of the restorations and microbiological analysis of dentin

Grant number: 16/21030-3
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2017
Effective date (End): August 31, 2018
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Silmara Aparecida Milori Corona
Grantee:Luísa Valente Gotardo Lara Alves
Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


This clinical study is to evaluate the antimicrobial photodynamic therapy (PDT) in caries in deciduous teeth, through clinical evaluation of restorations and microbiological analysis of dentin. The sample will be composed of children (n = 20) of both genders, between 6 and 8 years of age, which have active caries with cavitation reaching dentine, located on the occlusal surface (Class I) of deciduous molars counterparts. The selective removal of caries lesion will be performed, considering that, in the surrounding walls, will be removed all necrotic dentin and affected dentin (demineralized) by means of steel drills at low speed, and the pulp wall will be performed only the removal of necrotic dentin with curettes, maintaining the affected dentin. The deciduous molars counterparts of each child will be randomly assigned into two groups according to the treatment of carious lesions: 1) absent PDT (control) and 2) PDT. To Antimicrobial Photodynamic therapy will be use the laser InGaAIP low intensity, wavelength 660 nm, the red region of the light spectrum, power of 100mW and energy density of 640 J / cm2 (18 J) for 180 seconds, associated with the photosensitizer toluidine blue 0.01%.After treatment of the affected dentin will be performed extensive washing for 1 minute. For microbiology analysis (n=10), collection of the pulp wall dentine will be carried out in three periods: before treatment of carious lesions, after the selective removal of the lesion caries and after treatment of affected dentin. Pulp protection with calcium hydroxide cement and glass ionomer cement will be carried out and subsequently phosphoric acid etching to 35%. The deciduous molars will be restored with adhesive (Single Bond 2 - 3M) and composite resin (Z250 - 3M). For the clinical analysis of the restorations (n = 20), three calibrated and experienced professionals will use the criteria United States Public Health Service (USPHS) modified in periods: 7 days (after polishing) and 6 months after completion of the restorations. During the microbiological analysis, colony forming units (CFUs) will be counted and the results are expressed as CFU / mg of remaining dentin. The data will be analyzed to choose the appropriate statistical method. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GOTARDO LARA ALVES, LUISA VALENTE; CURYLOFO-ZOTTI, FABIANA ALMEIDA; BORSATTO, MARIA CRISTINA; DE SOUZA SALVADOR, SERGIO LUIZ; VALERIO, RODRIGO ALEXANDRE; SOUZA-GABRIEL, ALINE EVANGELISTA; MILORI CORONA, SILMARA APARECIDA. Influence of antimicrobial photodynamic therapy in carious lesion. Randomized split-mouth clinical trial in primary molars. Photodiagnosis and Photodynamic Therapy, v. 26, p. 124-130, . (16/21030-3)

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