Marine-derived fungus Aspergillus cf. tubingensis LAMAI 31: a new genetic resource for xylanase production

Autor(es)

dos Santos, Juliana A. ^[1] ; Vieira, Juliana M. F. ^[1] ; Videira, Alexandre ^[1] ; Meirelles, Lucas A. ^[1] ; Rodrigues, Andre ^[1] ; Taniwaki, Marta H. ^[2] ; Sette, Lara D. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Dept Bioquim & Microbiol, 24A, 1515, BR-13506900 Rio Claro, SP - Brazil

^[2] Inst Tecnol Alimentos ITAL, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AMB EXPRESS; v. 6, MAR 24 2016.
• Citações Web of Science: 4

Resumo

Marine-derived fungi have been reported as relevant producers of enzymes, which can have different properties in comparison with their terrestrial counterparts. The aim of the present study was to select from a collection of 493 marine-derived fungi the best producer of xylanase in order to evaluate the enzymatic production under different conditions. A total of 112 isolates produced xylanase in solid medium containing xylan as the carbon source, with 31 of them able to produce at least 10 U/mL of the enzyme. The best production (49.41 U/mL) was achieved by the strain LAMAI 31, identified as Aspergillus cf. tubingensis. After confirming the lack of pathogenicity (absence of ochratoxin A and fumonisin B-2 production) this fungus was submitted to the experimental design in order to evaluate the effect of different variables on the enzymatic production, with the aim of optimizing culture conditions. Three experimental designs (two Plackett-Burman and one factorial fractional) were applied. The best condition for the enzymatic production was defined, resulting in an increase of 12.7 times in comparison with the initial production during the screening experiments. In the validation assay, the peak of xylanase production (561.59 U/mL) was obtained after 96 h of incubation, being the best specific activity achieved after 72 h of incubation. Xylanase from A. cf. tubingensis LAMAI 31 had optimum pH and temperature at 5.0 and 55 degrees C, respectively, and was shown to be stable at a range of 40-50 degrees C, and in pH from 3.6 to 7.0. Results from the present work indicate that A. cf. tubingensis LAMAI 31 can be considered as a new genetic resource for xylanase production. (AU)

• Processo FAPESP: 11/16765-0 - Código de barras de DNA e potencial biotecnológico dos microfungos associados aos ninhos das formigas cortadeiras
• Beneficiário: André Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 10/17033-0 - Exploração biotecnológica de fungos derivados da Antártica
• Beneficiário: Lara Durães Sette
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/19486-0 - Biotecnologia marinha e Antártica: enzimas microbianas e suas aplicações
• Beneficiário: Lara Durães Sette
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/08338-0 - Sistemática e filogenia do fungo parasita Escovopsis associado às formigas Attini
• Beneficiário: Lucas Andrade Meirelles
• Modalidade de apoio: Bolsas no Brasil - Mestrado