Electric field-assisted solid phase extraction and cleanup of ionic compounds in complex food matrices: Fluoroquinolones in eggs

Autor(es)

Ribeiro, Cyntia Cabral ^[1] ; Orlando, Ricardo Mathias ^[2] ; Rodrigues Rohwedder, Jarbas Jose ^[3] ; Reyes Reyes, Felix Guillermo ^[1] ; Rath, Susanne ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Food Sci, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

^[2] Univ Fed Minas Gerais, Dept Chem, Belo Horizonte, MG - Brazil

^[3] Univ Estadual Campinas, Dept Analyt Chem, Inst Chem, POB 6154, BR-13084971 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 152, p. 498-503, MAY 15 2016.
• Citações Web of Science: 3

Resumo

The use of electric fields as additional driving forces in sample preparation techniques is an innovative approach that is environmentally friendly, straightforward, and able to overcome several limitations of conventional sample preparation procedures. In this work, the advantages of electric field-assisted solid phase extraction (E-SPE) using syringe-type cartridges were demonstrated for the extraction of four fluoroquinolones (FQs) in their anionic forms. The FQs were extracted from eggs and subsequently determined by UHPLC-MS/MS. The use of electric fields during the washing and final elution steps resulted in a significant improvement of the extraction efficiencies for almost all FQs when compared to conventional SPE. Intra- and inter-day assays showed coefficients of variation below 10%. The better cleanup also resulted in the appearance of less precipitated matter in the final eluate, as well as reduced matrix effects. The results showed that the electrophoretic forces derived from electric fields are a promising way of significantly increasing the extraction efficiency of ionic analytes, while minimizing matrix effects associated with complex samples. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/11401-3 - Avaliação da aplicação de potencial elétrico em extração em fase sólida na determinação de sulfonamidas em ovos por HPLC-DAD e LC-MS/MS
• Beneficiário: Cyntia Cabral Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 13/09543-7 - Resíduos de medicamentos veterinários no ambiente
• Beneficiário: Susanne Rath
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 08/03064-1 - Aplicação de potenciais elétricos em procedimentos de extração em fase sólida
• Beneficiário: Susanne Rath
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/57808-1 - Instituto Nacional de Ciências e Tecnologias Analíticas Avançadas - INCTAA
• Beneficiário: Celio Pasquini
• Modalidade de apoio: Auxílio à Pesquisa - Temático