Production of bacteriocin-like inhibitory substances (BLIS) by Bifidobacterium lactis using whey as a substrate

Autor(es)

Balciunas, Eduardo Marcos ^[1] ; Al Arni, Saleh ^[2] ; Converti, Attilio ^[3] ; Guy Leblanc, Jean ^[4] ; De Souza Oliveira, Ricardo Pinheiro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Ave Lineu Prestes 580, BR-13560 Sao Carlos - Brazil

^[2] King Saud Univ, Dept Chem Engn, POB 800, Riyadh 11421 - Saudi Arabia

^[3] Univ Genoa, Dept Civil Chem & Environm Engn, Pole Chem Engn, Via Opera Pia 15, Genoa - Italy

^[4] Ctr Referencia Lactobacillus CERELA CONICET, Chacabuco 145, San Miguel De Tucuman, Tucuman - Argentina

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY; v. 69, n. 2, p. 236-242, MAY 2016.
• Citações Web of Science: 4

Resumo

The objective of this work was to evaluate the production of bacteriocin-like inhibitory substances (BLIS) by Bifidobacterium animalis subsp. lactis in whey supplemented with yeast extract, inulin, Tween-80 or L-cysteine. Cell growth, acidification, glucose and lactose consumption as well as BLIS production were measured during fermentations carried out in shake flasks. The best additive for both cell growth and BLIS production was shown to be yeast extract, which gave the highest concentrations of biomass (9.9 log cfu/mL) and BLIS (800 AU/mL). In a bench-scale fermentor, B. lactis growth and BLIS production were between 6% and 25% higher than in flasks depending on the conditions assayed. (AU)

• Processo FAPESP: 13/12713-1 - Produção biotecnológica e purificação de plantaricina por Lactobacillus plantarum St16Pa utilizando meio de cultivo natural
• Beneficiário: Ricardo Pinheiro de Souza Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular