Anaesthetic efficacy of unilamellar and multilamellar liposomal formulations of articaine in inflamed and uninflamed tissue

Autor(es)

da Silva, Camila Batista ^[1] ; Groppo, Francisco Carlos ^[1] ; dos Santos, Cleiton Pita ^[1] ; Serpe, Luciano ^[1] ; Franz-Montan, Michelle ^[1] ; de Paula, Eneida ^[2] ; Ranali, Jose ^[1] ; Volpato, Maria Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Physiol Sci, Av Limeira, 901 Bairro Areiao, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Campinas UNICAMP, Inst Biol, Dept Biochem, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BRITISH JOURNAL OF ORAL & MAXILLOFACIAL SURGERY; v. 54, n. 3, p. 295-300, APR 2016.
• Citações Web of Science: 6

Resumo

We compared the efficacy of articaine encapsulated in multilamellar and unilamellar liposomes with that of articaine with epinephrine, after infiltration into inflamed and uninflamed tissue in rats. We encapsulated 4% articaine in multilamellar (articaine:multi) and unilamellar (articaine:uni) liposomes and compared them with 4% articaine with 1:100 000 epinephrine (articaine:epinephrine), in inflamed (plantar incision into the hind paw) and uninflamed (infraorbital nerve block) tissue in rats. Anaesthetic formulations (0.1 ml) were injected near the right infraorbital foramen in uninflamed tissue, where success and duration of anaesthesia were assessed by pinching the upper lip every 5 minutes. For inflamed tissue the anaesthetic formulations (0.1 ml) were injected laterally into a surgical wound made 24 hours earlier in the plantar region of the rat's right hind paw. The degree of anaesthesia was assessed by application of forces laterally to the wound with electronic von Frey filaments. Articaine:uni resulted in less successful anaesthesia than both articaine:multi (p=1.1x10(-5)) and articaine:epinephrine (p=4.3x10(-8)) in uninflamed tissue, but there were no differences in duration or success of anaesthesia between articaine:epinephrine and articaine:multi. In inflamed tissue articaine:epinephrine gave significantly more effective anaesthesia for longer than articaine:uni (p=2.3x10(-6)), and articaine:epinephrine (p=1.8x10(-6)) formulations, which did not differ from each other. Multilamellar liposomal articaine could be an option for local anaesthesia in uninflamed tissues. However, articaine with epinephrine gave better results than liposomal formulations in inflamed tissue. (C) 2016 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 06/00121-9 - Novas formulações de anestésicos locais de liberação controlada: do desenvolvimento ao teste clínico odontológico
• Beneficiário: Eneida de Paula
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 09/11842-7 - Eficácia anestésica das preparações lipossomais uni e multilamelar de articaína, em bloqueio dos nervos infraorbital e alveolar inferior e em ferida cirúrgica, em ratos.
• Beneficiário: Camila Batista da Silva de Araujo Candido
• Modalidade de apoio: Bolsas no Brasil - Mestrado