Morphological, ultrastructural and phylogenetic analyses of Myxobolus hilarii n. sp (Myxozoa, Myxosporea), a renal parasite of farmed Brycon hilarii in Brazil

Autor(es)

Capodifoglio, Kassia R. H. ^[1] ; Adriano, Edson A. ^{[2, 3]} ; Milanin, Tiago ^[3] ; Silva, Marcia R. M. ^[1] ; Maia, Antonio A. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Med Vet, Rua Duque Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Fed Sao Paulo UNIFESP, Dept Ciencias Biol, Rua Prof Artur Riedel 275, BR-09972270 Diadema, SP - Brazil

^[3] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Biol Anim, Caixa Postal 6109, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Parasitology International; v. 65, n. 3, p. 184-190, JUN 2016.
• Citações Web of Science: 11

Resumo

Myxobolus hilarii n. sp. was described, based on morphology, histology, ultrastructure and 18S rDNA sequencing, infecting the kidney of Borcon hilarii (Valenciennes 1850) (Characiformes: Bryconidae) taken from fish farms in the state of Sao Paulo, Brazil. Thirteen specimens of B. hilarii were examined and 100% had round, white plasmodia in the kidney. The mature myxospores were rounded, measuring 11.5 +/- 0.8 (9.8-13.4) mu m in length, 11.0 +/- 0.7 (9.7-12.4) mu m in width and 7.6 +/- 1.0 (6.7-9.0) mu m in thickness. Polar capsules were elongated and of equal size, with 6.5 +/- 0.4 (6.0-7.2) mu m in length and 4.0 +/- 02 (3.6-53) mu m in width and their polar filaments had 5 to 7 coils. Histological analysis revealed plasmodial development in the renal tubules, causing compression and deformation of adjacent tissues and destruction of renal tubule cells. Ultrastructural analysis showed direct contact between the plasmodial wall and the host tissue and asynchronous plasmodial development. The phylogenetic analysis of South American myxobolids, based on 18S rDNA sequencing, showed the myxosporeans grouping into two main clades. M. hilarii n. sp. appears as sister species of Myxobolus piraputangae. (C) 2015 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 06/59075-6 - Mixosporídeos parasitos de Pseudoplatystoma corruscans (pintado), Pseudoplatystoma fasciatum (cachara), Salminus brasiliensis (dourado) e Brycon hilari (piraputanga) oriundos de ambiente natural e de sistemas de criação: taxonomia e interação parasito-hospedeiro
• Beneficiário: Edson Aparecido Adriano
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/08549-6 - Avaliação do envolvimento de hospedeiros invertebrados no ciclo de vida de Myxozoa parasitos de pintado (incluindo híbridos) e pacu (incluindo híbridos) de sistemas de criação.
• Beneficiário: Tiago Milanin
• Modalidade de apoio: Bolsas no Brasil - Doutorado