Pre-mRNA splicing is modulated by antifungal drugs in the filamentous fungus Neurospora crassa

Autor(es)

Mendes, Niege S. ^[1] ; Silva, Patricia M. ^[1] ; Silva-Rocha, Rafael ^[2] ; Martinez-Rossi, Nilce M. ^[1] ; Rossi, Antonio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Mol & Cellular Biol, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FEBS OPEN BIO; v. 6, n. 4, p. 358-368, APR 2016.
• Citações Web of Science: 5

Resumo

For this study, we sought to identify pre-mRNA processing events modulated by changes in extracellular pH, inorganic phosphate, and antifungal drugs. We examined genes with at least four putative introns whose transcriptional level responded to these effectors. We showed that the intron retention levels of genes encoding asparagine synthetase 2, C6-zinc finger regulator (fluffy), and a farnesyltransferase respond to amphotericin B, ketoconazole, and other effectors. In general, the assayed antifungals promoted the disruption of the structural domains of these proteins probably leading to their inactivation, which emphasize the complexity of the metabolic modulation exerted by antifungal signaling. (AU)

• Processo FAPESP: 12/03689-7 - Mecanismos moleculares envolvidos no sensoriamento de fosfato inorgânico (Pi) em Neurospora crassa e em outros fungos.
• Beneficiário: Patrícia Martins da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/03847-7 - Caracterização molecular de mecanismos envolvidos na patogenicidade e sinalização celular em fungos
• Beneficiário: Nilce Maria Martinez-Rossi
• Modalidade de apoio: Auxílio à Pesquisa - Temático