Expression of ATP6V1C1 during oral carcinogenesis

Autor(es)

Alves, M. G. Oliveira ^{[1, 2]} ; Carta, C. F. L. ^[1] ; Padin-Iruegas, M-E ^[3] ; Perez-Sayans, M. ^[4] ; Suarez-Penaranda, J. M. ^{[5, 6]} ; Issa, J. S. ^[7] ; Garcia-Garcia, A. ^[4] ; Almeida, J. D. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP - Brazil

^[2] Univ Braz Cubas, Mogi Das Cruzes, SP - Brazil

^[3] Univ Vigo, Dept Funct Biol & Hlth Sci, Human Anat & Embryol Area, Pontevedra - Spain

^[4] Fac Med & Dent, Oral Surg & Implantol Unit, Oral Med, Santiago De Compostela - Spain

^[5] Univ Hosp, Dept Pathol & Forens Sci, Santiago De Compostela - Spain

^[6] Sch Med Santiago de Compostela, Santiago De Compostela - Spain

^[7] Univ Sao Paulo, Sch Med, Hosp Clin, Smoking Cessat Program, Area Cardiol, Heart Inst, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOTECHNIC & HISTOCHEMISTRY; v. 91, n. 4, p. 263-268, 2016.
• Citações Web of Science: 0

Resumo

We investigated the gene and protein expressions of V-type ATPase protein subunit C1 (ATP6V1C1) in cases of oral squamous cell carcinoma (OSCC) and contralateral normal mucosa in smokers, nonsmokers and former smokers. Subjects were separated into five groups of 15: group 1, smokers with OSCC; group 2, normal contralateral mucosa of OSCC patients; group 3, chronic smokers; group 4, former smokers who had stopped smoking 1 year earlier; group 5, individuals who had never smoked. Exfoliative cytology specimens from oral mucosa of smokers, former smokers and nonsmokers showed normal gene and protein expression. We found significantly greater gene expression in the OSCC group than in the nonsmoker groups. No difference in gene expression was observed between normal contralateral mucosa and nonsmoker groups, smoker and nonsmoker groups or former smoker and nonsmoker groups. We observed intense immunostaining for ATP6V1C1 protein in all cases of OSCC and weak or no staining in smoker, former smoker and nonsmoker groups. Significantly greater expression of ATP6V1C1 protein was observed in the OSCC group compared to the other groups, which supports the role of ATP6V1C1 in effecting changes associated with oral cancer. Analysis of the mucosae of chronic smokers, former smokers and the normal contralateral mucosa of patients with OSCC showed unaltered ATP6V1C1 gene and protein expression. Early stages of carcinogenesis, represented by altered epithelium of chronic smokers, had neither gene nor protein alterations as seen in OSCC. Therefore, we infer that the changes in ATP6V1C1 occur during later stages of carcinogenesis. Our preliminary study provides a basis for future studies of using ATP6V1C1 levels for detecting early stage OSCC. (AU)

• Processo FAPESP: 13/06251-5 - Estudo dos efeitos causados pelo tabagismo crônico em células mucosa bucal
• Beneficiário: Mônica Ghislaine Oliveira Alves
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/05371-4 - Avaliação citogenética dos danos causados pelo tabagismo à mucosa bucal
• Beneficiário: Janete Dias Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Regular