Trypanosoma cruzi: single cell live imaging inside infected tissues

Autor(es)

Ferreira, Bianca Lima ^[1] ; Orikaza, Cristina Mary ^[1] ; Cordero, Esteban Mauricio ^[1] ; Mortara, Renato Arruda ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo EPM UNIFESP, Dept Microbiol Imunol & Parasitol, Rua Botucatu 862 6th Floor, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cellular Microbiology; v. 18, n. 6, p. 779-783, JUN 2016.
• Citações Web of Science: 2

Resumo

Although imaging the live Trypanosoma cruzi parasite is a routine technique in most laboratories, identification of the parasite in infected tissues and organs has been hindered by their intrinsic opaque nature. We describe a simple method for in vivo observation of live single-cell Trypanosoma cruzi parasites inside mammalian host tissues. BALB/c or C57BL/6 mice infected with DsRed-CL or GFP-G trypomastigotes had their organs removed and sectioned with surgical blades. Ex vivo organ sections were observed under confocal microscopy. For the first time, this procedure enabled imaging of individual amastigotes, intermediate forms and motile trypomastigotes within infected tissues of mammalian hosts. (AU)

• Processo FAPESP: 14/21338-2 - Avaliação in loco da infecção aguda e crônica por tripomastigotas de Trypanosoma cruzi (TCI e TcVI) em camundongos isogênicos BALB/c e C57BL/6
• Beneficiário: Bianca Rodrigues Lima Ferreira
• Modalidade de apoio: Bolsas no Brasil - Mestrado