Sex differences on solid organ histological characteristics after brain death1

Autor(es)

Raif Restivo Simão ^[1] ; Sueli Gomes Ferreira ^[2] ; Guilherme Konishi Kudo ^[3] ; Roberto Armstrong Junior ^[4] ; Luiz Fernando Ferraz da Silva ^[5] ; Paulina Sannomiya ^[6] ; Ana Cristina Breithaupt-Faloppa ^[7] ; Luiz Felipe Pinho Moreira ^[8]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. School of Medicine - Brasil

^[2] USP. School of Medicine - Brasil

^[3] USP. School of Medicine - Brasil

^[4] USP. School of Medicine - Brasil

^[5] USP. School of Medicine - Brasil

^[6] USP. School of Medicine - Brasil

^[7] USP. School of Medicine - Brasil

^[8] USP. School of Medicine - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Acta Cirurgica Brasileira; v. 31, n. 4, p. 278-285, 2016-04-00.

Resumo

PURPOSE: To investigate gender differences in the evolution of the inflammatory process in rats subjected to brain death (BD). METHODS: Adult Wistar rats were divided into three groups: female; ovariectomized female; and male rats. BD was induced using intracranial balloon inflation and confirmed by maximal pupil dilatation, apnea, absence of reflex, and drop of mean arterial pressure. Six hours after BD, histological evaluation was performed in lungs, heart, liver and kidneys, and levels of inflammatory proteins, estrogen, progesterone, and corticosterone were determined in plasma. RESULTS: In the lungs, females presented more leukocyte infiltration compared to males (p<0.01). Ovariectomized female rat lungs were more hemorrhagic compared to other groups (p<0.001). In the heart, females had higher leukocyte infiltration and tissue edema compared to males (p<0.05). In the liver and kidneys, there were no differences among groups. In female group estradiol and progesterone were sharply reduced 6 hours after BD (p<0.001) to values observed in ovariectomized females and males. Corticosterone levels were similar. CONCLUSIONS: Sex hormones influence the development of inflammation and the status of organs. The increased inflammation in lungs and heart of female rats might be associated with the acute reduction in female hormones triggered by BD. (AU)

• Processo FAPESP: 13/20282-0 - Influência do gênero nas alterações microcirculatórias e no processo inflamatório em diferentes órgãos em modelo de morte encefálica em ratos
• Beneficiário: Ana Cristina Breithaupt Faloppa
• Modalidade de apoio: Auxílio à Pesquisa - Regular