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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The MqsRA Toxin-Antitoxin System from Xylella fastidiosa Plays a Key Role in Bacterial Fitness, Pathogenicity, and Persister Cell Formation

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Autor(es):
Merfa, Marcus V. [1, 2] ; Niza, Barbara [2, 1] ; Takita, Marco A. [2] ; De Souza, Alessandra A. [2]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Dept Genet Evolucao & Bioagentes, Campinas, SP - Brazil
[2] Ctr Citricultura Sylvio Moreira, Inst Agron, Cordeiropolis - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN MICROBIOLOGY; v. 7, JUN 10 2016.
Citações Web of Science: 16
Resumo

Through the formation of persister cells, bacteria exhibit tolerance to multidrug and other environmental stresses without undergoing genetic changes. The toxin-antitoxin (TA) systems are involved in the formation of persister cells because they are able to induce cell dormancy. Among the TA systems, the MqsRA system has been observed to be highly induced in persister cells of Xylella fastidiosa (causal agent of citrus variegated chlorosis-CVC) activated by copper stress, and has been described in Escherichia coil as related to the formation of persister cells and biofilms. Thus, we evaluated the role of this TA system in X. fastidiosa by overexpressing the MqsR toxin, and verified that the toxin positively regulated biofilm formation and negatively cell movement, resulting in reduced pathogenicity in citrus plants. The overexpression of MqsR also increased the formation of persister cells under copper stress. Analysis of the gene and protein expression showed that this system likely has an autoregulation mechanism to express the toxin and antitoxin in the most beneficial ratio for the cell to oppose stress. Our results suggest that this TA system plays a key role in the adaptation and survival of X fastidiosa and reveal new insights into the physiology of phytopathogen host interactions. (AU)

Processo FAPESP: 10/50712-9 - Papel biologico do modulo toxina-antitoxina no mecanismo de morte celular programada em xylella fastidiosa.
Beneficiário:Alessandra Alves de Souza
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/17485-7 - Estudo funcional de genes associados à defesa de plantas a fitopatógenos: foco no controle de Xylella fastidiosa, agente causal da clorose variegada dos citros
Beneficiário:Alessandra Alves de Souza
Linha de fomento: Auxílio à Pesquisa - Regular