A case study in flux balance analysis: Lysine, a cephamycin C precursor, can also increase clavulanic acid production

Autor(es)

Cavallieri, Andre Pastrelo ^[1] ; Baptista, Amanda Salvador ^[1] ; Leite, Carla Andrea ^[1] ; Gonsales da Costa Araujo, Maria Lucia ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Sso Paulo State Univ, Inst Chem, Dept Biochem & Technol Chem, Prof Francisco Degni St 55, BR-14800900 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biochemical Engineering Journal; v. 112, p. 42-53, AUG 15 2016.
• Citações Web of Science: 2

Resumo

Streptomyces clavuligerus is an excellent model for studying complex relationships involved in the biosynthesis of secondary metabolites. Despite the fact that several studies have investigated the mechanisms pertaining to the biosynthesis of two bioactive compounds produced by S. clavuligerus, cephamycin C (CephC) and clavulanic acid (CA), few have focused on metabolic flux analyses of this microorganism. It was proposed a novel representative metabolic model for S. clavuligerus addressing the simultaneous production of CephC and CA using flux balance analysis methodology (FBA). Although both are synthesized via different pathway, flux analysis showed that the production of these bioactives are connected. CephC production showed high dependency on N metabolism while CA production is more related with C metabolism. Adding lysine plus maltose increased CA, an interesting outcome since lysine is a CephC precursor. Notwithstanding, it was possible to propose a mechanism for this behavior by means of flux analysis. Furthermore, the results suggest that ATP yield maximization is the objective function that best suits the conditions under investigation. (c) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/22181-4 - Monitoramento de cultivos submersos de linhagens de Streptomyces clavuligerus para a quantificação de distribuição de fluxos metabólicos
• Beneficiário: Amanda Salvador Baptista
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/02632-4 - Análise de fluxos metabólicos para a otimização in silico da produção de bioativos por Streptomyces clavuligerus
• Beneficiário: Maria Lucia Gonsales da Costa Araujo
• Linha de fomento: Auxílio à Pesquisa - Regular