INHIBITION OF THE PONTINE KOLLIKER-FUSE NUCLEUS REDUCES GENIOGLOSSAL ACTIVITY ELICITED BY STIMULATION OF THE RETROTRAPEZOID CHEMORECEPTOR NEURONS

Autor(es)

Silva, Josiane N. ^[1] ; Lucena, Elvis V. ^[2] ; Silva, Talita M. ^[2] ; Damasceno, Roselia S. ^[2] ; Takakura, Ana C. ^[1] ; Moreira, Thiago S. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Neuroscience; v. 328, p. 9-21, JUL 22 2016.
• Citações Web of Science: 8

Resumo

The Kolliker-Fuse (KF) region, located in the dorsolateral pons, projects to several brainstem areas involved in respiratory regulation, including the chemoreceptor neurons within the retrotrapezoid nucleus (RTN). Several lines of evidence indicate that the pontine KF region plays an important role in the control of the upper airways for the maintenance of appropriate airflow to and from the lungs. Specifically, we hypothesized that the KF region is involved in mediating the response of the hypoglossal motor activity to central respiratory chemoreflex activation and to stimulation of the chemoreceptor neurons within the RTN region. To test this hypothesis, we combined immunohistochemistry and physiological experiments. We found that in the KF, the majority of biotinylated dextran amine (BDA)-labeled axonal varicosities contained detectable levels of vesicular glutamate transporter-2 (VGLUT2), but few contained glutamic acid decarboxylase-67 (GAD67). The majority of the RTN neurons that were FluorGold (FG)-immunoreactive (i.e., projected to the KF) contained hypercapnia-induced Fos, but did not express tyrosine hydroxylase. In urethane-anesthetized sino-aortic denervated and vagotomized male Wistar rats, hypercapnia (10% CO2) or N-methyl-D-aspartate (NMDA) injection (0.1 mM) in the RTN increased diaphragm (Dia(EMG)) and genioglossus muscle (GG(EMG)) activities and elicited abdominal (Abd(EMG)) activity. Bilateral injection of muscimol (GABA-A agonist; 2 mM) into the KF region reduced the increase in DiaEMG and GG(EMG) produced by hypercapnia or NMDA into the RTN. Our data suggest that activation of chemoreceptor neurons in the RTN produces a significant increase in the genioglossus muscle activity and the excitatory pathway is dependent on the neurons located in the dorsolateral pontine KF region. (C) 2016 IBRO. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/03568-5 - Projeções anatômicas existentes entre o núcleo retrotrapezóide e o grupo respiratório ventrolateral caudal
• Beneficiário: Josiane do Nascimento Silva
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/09776-3 - Mecanismos neurais envolvidos na geração do ritmo expiratório: possível envolvimento do núcleo retrotrapezoide e da região parafacial
• Beneficiário: Ana Carolina Takakura Moreira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 13/10573-8 - Mecanismos neurais da superfície ventral do bulbo envolvidos na quimiorrecepção
• Beneficiário: Thiago dos Santos Moreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/54888-7 - Mecanismos neurais envolvidos na quimiorrecepção
• Beneficiário: Eduardo Colombari
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/00401-5 - Integração entre o bulbo ventrolateral rostral e o núcleo paraventricular do hipotálamo durante a ativação dos quimiorreceptores arteriais: possível envolvimento dos mecanismos catecolaminérgicos
• Beneficiário: Talita de Melo e Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/15692-7 - Envolvimento do núcleo Kolliker-Fuse no controle cardiorrespiratório durante a ativação dos quimiorreceptores centrais e periféricos
• Beneficiário: Rosélia dos Santos Damasceno
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/16166-2 - Participação do grupamento parafacial/núcleo retrotrapezóide no controle de vias aéreas superiores
• Beneficiário: Elvis Viana de Lucena
• Modalidade de apoio: Bolsas no Brasil - Mestrado