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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

INHIBITION OF THE PONTINE KOLLIKER-FUSE NUCLEUS REDUCES GENIOGLOSSAL ACTIVITY ELICITED BY STIMULATION OF THE RETROTRAPEZOID CHEMORECEPTOR NEURONS

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Author(s):
Silva, Josiane N. [1] ; Lucena, Elvis V. [2] ; Silva, Talita M. [2] ; Damasceno, Roselia S. [2] ; Takakura, Ana C. [1] ; Moreira, Thiago S. [2]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Neuroscience; v. 328, p. 9-21, JUL 22 2016.
Web of Science Citations: 8
Abstract

The Kolliker-Fuse (KF) region, located in the dorsolateral pons, projects to several brainstem areas involved in respiratory regulation, including the chemoreceptor neurons within the retrotrapezoid nucleus (RTN). Several lines of evidence indicate that the pontine KF region plays an important role in the control of the upper airways for the maintenance of appropriate airflow to and from the lungs. Specifically, we hypothesized that the KF region is involved in mediating the response of the hypoglossal motor activity to central respiratory chemoreflex activation and to stimulation of the chemoreceptor neurons within the RTN region. To test this hypothesis, we combined immunohistochemistry and physiological experiments. We found that in the KF, the majority of biotinylated dextran amine (BDA)-labeled axonal varicosities contained detectable levels of vesicular glutamate transporter-2 (VGLUT2), but few contained glutamic acid decarboxylase-67 (GAD67). The majority of the RTN neurons that were FluorGold (FG)-immunoreactive (i.e., projected to the KF) contained hypercapnia-induced Fos, but did not express tyrosine hydroxylase. In urethane-anesthetized sino-aortic denervated and vagotomized male Wistar rats, hypercapnia (10% CO2) or N-methyl-D-aspartate (NMDA) injection (0.1 mM) in the RTN increased diaphragm (Dia(EMG)) and genioglossus muscle (GG(EMG)) activities and elicited abdominal (Abd(EMG)) activity. Bilateral injection of muscimol (GABA-A agonist; 2 mM) into the KF region reduced the increase in DiaEMG and GG(EMG) produced by hypercapnia or NMDA into the RTN. Our data suggest that activation of chemoreceptor neurons in the RTN produces a significant increase in the genioglossus muscle activity and the excitatory pathway is dependent on the neurons located in the dorsolateral pontine KF region. (C) 2016 IBRO. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 12/03568-5 - Anatomic projections between retrotrapezoid nucleus and caudal ventral respiratory group
Grantee:Josiane do Nascimento Silva
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 10/09776-3 - Neural mechanisms involved in expiratory rhythm generator: possible involvement of the retrotrapezoid nucleus and the parafacial region
Grantee:Ana Carolina Takakura Moreira
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 13/10573-8 - Ventral surface chemoreceptor neuronal mechanisms
Grantee:Thiago dos Santos Moreira
Support Opportunities: Regular Research Grants
FAPESP's process: 09/54888-7 - Neural mechanisms involved on chemoreception
Grantee:Eduardo Colombari
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/00401-5 - Integration between rostroventrolateral medulla and paraventricular nucleus during arterial chemoreceptor activation: possible involvement of catecholaminergic mechanisms
Grantee:Talita de Melo e Silva
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 10/15692-7 - Involvement of the Kolliker-Fuse in cardiorespiratory control during activation of central and peripheral chemoreceptors
Grantee:Rosélia dos Santos Damasceno
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/16166-2 - Involvement of the parafacial/retrotrapezoid nucleus in the control of upper airway
Grantee:Elvis Viana de Lucena
Support Opportunities: Scholarships in Brazil - Master