In vivo photodynamic inactivation of Candida albicans using chloro-aluminum phthalocyanine

Autor(es)

Carmello, J. C. ^[1] ; Alves, F. ^[1] ; Ribeiro, A. P. D. ^[2] ; Basso, F. G. ^[3] ; de Souza Costa, C. A. ^[3] ; Tedesco, A. C. ^[4] ; Primo, F. L. ^[5] ; Mima, E. G. ^[1] ; Pavarina, A. C. ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Dept Dent Mat & Prosthodont, Araraquara Dent Sch, Araraquara - Brazil

^[2] UnB Univ Brasilia, Dept Dent, Sch Hlth Sci, Brasilia, DF - Brazil

^[3] UNESP Univ Estadual Paulista, Dept Physiol & Pathol, Araraquara Dent Sch, Araraquara - Brazil

^[4] Univ Sao Paulo, Ctr Nanotechnol & Tissue Engineers, FFCLRP, Photobiol & Photomed Res Grp, Ribeirao Preto - Brazil

^[5] UNESP Univ Estadual Paulista, Dept Bioproc & Biotechnol, Sch Pharmaceut Sci, Araraquara - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ORAL DISEASES; v. 22, n. 5, p. 415-422, JUL 2016.
• Citações Web of Science: 6

Resumo

This study evaluated the photoinactivation of Candida albicans in a murine model of oral candidiasis using chloro-aluminum phthalocyanine (ClAlP) encapsulated in cationic nanoemulsions (NE) and chloro-aluminum phthalocyanine (ClAlP) diluted in DMSO (DMSO) as photosensitizer (PS). Seventy-five 6-week-old female Swiss mice were immunosuppressed and inoculated with C.albicans to induce oral candidiasis. PDT was performed on the tongue by the application of the photosensitizers and LED light (100Jcm(-2)-660nm). Twenty-four hours and 7days after treatments, microbiological evaluation was carried out by recovering C.albicans from the tongue of animals (CFUml(-1)). Then, mice were sacrificed and the tongues were surgically removed for histological and biomolecular analysis of pro- and anti-inflammatory cytokines. Data were analyzed by ANOVA followed by Tukey's post hoc test. ClAlP-NE-mediated PDT reduced 2.26 log(10) of C.albicans recovered from the tongue when compared with the control group (P-L-) (P<0.05). PDT did not promote adverse effects on the tongue tissue. Seven days after treatment, all animals were completely healthy. In summary, PDT mediated by chloro-aluminum phthalocyanine entrapped in cationic nanoemulsions was effective in reducing C.albicans recovered from the oral lesions of immunocompromised mice. (AU)

• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 12/17468-2 - Estudo do efeito da Terapia Fotodinâmica Antimicrobiana no tratamento de candidose oral induzida em língua de camundongo. Avaliação in vivo de um hidrogel de Photodithazine e nanoemulsão catiônica de Cloro Alumínio Ftalocianina .
• Beneficiário: Juliana Cabrini Carmello
• Modalidade de apoio: Bolsas no Brasil - Doutorado