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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vivo photodynamic inactivation of Candida albicans using chloro-aluminum phthalocyanine

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Author(s):
Carmello, J. C. [1] ; Alves, F. [1] ; Ribeiro, A. P. D. [2] ; Basso, F. G. [3] ; de Souza Costa, C. A. [3] ; Tedesco, A. C. [4] ; Primo, F. L. [5] ; Mima, E. G. [1] ; Pavarina, A. C. [1]
Total Authors: 9
Affiliation:
[1] UNESP Univ Estadual Paulista, Dept Dent Mat & Prosthodont, Araraquara Dent Sch, Araraquara - Brazil
[2] UnB Univ Brasilia, Dept Dent, Sch Hlth Sci, Brasilia, DF - Brazil
[3] UNESP Univ Estadual Paulista, Dept Physiol & Pathol, Araraquara Dent Sch, Araraquara - Brazil
[4] Univ Sao Paulo, Ctr Nanotechnol & Tissue Engineers, FFCLRP, Photobiol & Photomed Res Grp, Ribeirao Preto - Brazil
[5] UNESP Univ Estadual Paulista, Dept Bioproc & Biotechnol, Sch Pharmaceut Sci, Araraquara - Brazil
Total Affiliations: 5
Document type: Journal article
Source: ORAL DISEASES; v. 22, n. 5, p. 415-422, JUL 2016.
Web of Science Citations: 6
Abstract

This study evaluated the photoinactivation of Candida albicans in a murine model of oral candidiasis using chloro-aluminum phthalocyanine (ClAlP) encapsulated in cationic nanoemulsions (NE) and chloro-aluminum phthalocyanine (ClAlP) diluted in DMSO (DMSO) as photosensitizer (PS). Seventy-five 6-week-old female Swiss mice were immunosuppressed and inoculated with C.albicans to induce oral candidiasis. PDT was performed on the tongue by the application of the photosensitizers and LED light (100Jcm(-2)-660nm). Twenty-four hours and 7days after treatments, microbiological evaluation was carried out by recovering C.albicans from the tongue of animals (CFUml(-1)). Then, mice were sacrificed and the tongues were surgically removed for histological and biomolecular analysis of pro- and anti-inflammatory cytokines. Data were analyzed by ANOVA followed by Tukey's post hoc test. ClAlP-NE-mediated PDT reduced 2.26 log(10) of C.albicans recovered from the tongue when compared with the control group (P-L-) (P<0.05). PDT did not promote adverse effects on the tongue tissue. Seven days after treatment, all animals were completely healthy. In summary, PDT mediated by chloro-aluminum phthalocyanine entrapped in cationic nanoemulsions was effective in reducing C.albicans recovered from the oral lesions of immunocompromised mice. (AU)

FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
Grantee:Vanderlei Salvador Bagnato
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 12/17468-2 - Effect of Antimicrobial Photodynamic Therapy in a murine model of oral candidosis. In vivo evaluation of a Photodithazine hydrogel and Aluminum-Chlorine-Phthalocyanine cationic nanoemulsion.
Grantee:Juliana Cabrini Carmello
Support Opportunities: Scholarships in Brazil - Doctorate