A fast method for bisphenol A and six analogues (S, F, Z, P, AF, AP) determination in urine samples based on dispersive liquid-liquid microextraction and liquid chromatography-tandem mass spectrometry

Autor(es)

Rocha, Bruno Alves ^[1] ; Brandao da Costa, Bruno Ruiz ^[1] ; Perez de Albuquerque, Nayara Cristina ^[2] ; Moraes de Oliveira, Anderson Rodrigo ^[2] ; Oliveira Souza, Juliana Maria ^[1] ; Al-Tameemi, Maha ^[3] ; Campiglia, Andres Dobal ^[3] ; Barbosa, Jr., Fernando ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Toxicol & Essencialidade Metais, Fac Ciencias Farmaceut Ribeirao Preto, Ave Cafe S-N, BR-14049903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14049901 Ribeirao Preto, SP - Brazil

^[3] Univ Cent Florida, Dept Chem, Orlando, FL 32816 - USA

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 154, p. 511-519, JUL 1 2016.
• Citações Web of Science: 39

Resumo

In this study, a novel method combining dispersive liquid-liquid microextraction (DLLME) and fast liquid chromatography coupled to mass spectrometry (LC-MS/MS) was developed and validated for the extraction and determination of bisphenol A (BPA) and six bisphenol analogues, namely bisphenol S (BPS), bisphenol F (BPF), bisphenol P (BPP), bisphenol Z (BPZ), bisphenol AP (BPAP) and bisphenol AF (BPAF) in human urine samples. Type and volume of extraction and disperser solvents, pH sample, ionic strength, and agitation were evaluated. The matrix -matched calibration curves of all analytes were linear with correlation coefficients higher than 0.99 in the range level of 0.5-20.0 ng The relative standard deviation (RSD), precision, at three concentrations (1.0, 8.0 and 15.0 ng mL(-1)) was lower than 15% with accuracy ranging from 90 to 112%. The biomonitoring capability of the new method was confirmed with the analysis of 50 human urine samples randomly collected from Brazilians. BPA was detected in 92% of the analyzed samples at concentrations ranging < LOQ to 10.4 ng mL(-1). The detection rates of BPS (10%), BPAF (4%) and BPF (2%) were much lower than 92%. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/23710-3 - Uso da espectroscopia Shpol'skii e LC-MS/MS para a determinação do bisfenol A e análogos em amostras de urina e avaliação da exposição em crianças residentes no estado de São Paulo
• Beneficiário: Bruno Alves Rocha
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado