Bacterial spores as particulate carriers for gene gun delivery of plasmid DNA

Autor(es)

Aps, Luana R. M. M. ^[1] ; Tavares, Milene B. ^[1] ; Rozenfeld, Julio H. K. ^[2] ; Lamy, M. Teresa ^[2] ; Ferreira, Luis C. S. ^[1] ; Diniz, Mariana O. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Vaccine Dev Lab, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Phys, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biotechnology; v. 228, p. 58-66, JUN 20 2016.
• Citações Web of Science: 3

Resumo

Bacillus subtilis spores represent a suitable platform for the adsorption of proteins, enzymes and viral particles at physiological conditions. In the present work, we demonstrate that purified spores can also adsorb DNA on their surface after treatment with cationic molecules. In addition, we demonstrate that DNA coated B. subtilis spores can be used as particulate carriers and act as an alternative to gold microparticles for the biolistic (gene gun) administration of plasmid DNA in mice. Gene gun delivery of spores pre-treated with DODAB (dioctadecyldimethylammonium bromide) allowed efficient plasmid DNA absorption and induced protein expression levels similar to those obtained with gold microparticles. More importantly, we demonstrated that a DNA vaccine adsorbed on spores can be loaded into biolistic cartridges and efficiently delivered into mice, which induced specific cellular and antibody responses. Altogether, these data indicate that B. subtilis spores represent a simple and low cost alternative for the in vivo delivery of DNA vaccines by the gene gun technology. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/13805-1 - Efeito adjuvante de esporos de Bacillus subtilis coadministrados a vacinas de DNA
• Beneficiário: Luana Raposo de Melo Moraes Aps
• Linha de fomento: Bolsas no Brasil - Mestrado