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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bacterial spores as particulate carriers for gene gun delivery of plasmid DNA

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Author(s):
Aps, Luana R. M. M. [1] ; Tavares, Milene B. [1] ; Rozenfeld, Julio H. K. [2] ; Lamy, M. Teresa [2] ; Ferreira, Luis C. S. [1] ; Diniz, Mariana O. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Vaccine Dev Lab, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Phys, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Biotechnology; v. 228, p. 58-66, JUN 20 2016.
Web of Science Citations: 3
Abstract

Bacillus subtilis spores represent a suitable platform for the adsorption of proteins, enzymes and viral particles at physiological conditions. In the present work, we demonstrate that purified spores can also adsorb DNA on their surface after treatment with cationic molecules. In addition, we demonstrate that DNA coated B. subtilis spores can be used as particulate carriers and act as an alternative to gold microparticles for the biolistic (gene gun) administration of plasmid DNA in mice. Gene gun delivery of spores pre-treated with DODAB (dioctadecyldimethylammonium bromide) allowed efficient plasmid DNA absorption and induced protein expression levels similar to those obtained with gold microparticles. More importantly, we demonstrated that a DNA vaccine adsorbed on spores can be loaded into biolistic cartridges and efficiently delivered into mice, which induced specific cellular and antibody responses. Altogether, these data indicate that B. subtilis spores represent a simple and low cost alternative for the in vivo delivery of DNA vaccines by the gene gun technology. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 11/13805-1 - Adjuvant effect of Bacillus subtilis spores co-administered with DNA vaccines
Grantee:Luana Raposo de Melo Moraes Aps
Support Opportunities: Scholarships in Brazil - Master