Bioaugmentation strategy employing a microbial consortium immobilized in chitosan beads for oil degradation in mesocosm scale

Autor(es)

Dellagnezze, B. M. ^[1] ; Vasconcellos, S. P. ^[2] ; Angelim, A. L. ^[3] ; Melo, V. M. M. ^[3] ; Santisi, S. ^[4] ; Cappello, S. ^[4] ; Oliveira, V. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Div Microbial Resources, Res Ctr Chem Biol & Agr CPQBA, CP 6171, BR-13081970 Campinas, SP - Brazil

^[2] Fed Univ Sao Paulo UNIFESP, Rua Prof Artur Riedel 275, BR-09972270 Diadema, SP - Brazil

^[3] Univ Fed Ceara, Lembiotech UFC, Av Humberto Monte 2977, Campus Pici, Bloco 909, BR-60455000 Fortaleza, CE - Brazil

^[4] CNR, Inst Coastal Marine Environm IAMC, Messina - Italy

• Tipo de documento: Artigo Científico
• Fonte: Marine Pollution Bulletin; v. 107, n. 1, p. 107-117, JUN 15 2016.
• Citações Web of Science: 10

Resumo

A bacterial consortium composed by four metagenomic clones and Bacillus subtilis strain CBMAI 707, all derived froth petroleum reservoirs, was entrapped in chitosan beads and evaluated regarding hydrocarbon degradation capability. Experiments were carried out in mesocosm scale (3000 L) with seawater artificially polluted with crude oil.At different time intervals, mesocosms were sampled and subjected to GC-FID and microbiological analyses, as total and heterotrophic culturable bacterial abundance (DAPI and CFLI count), biological oxygen demand (BOD) and taxonomic diversity (massive sequencing of 16S rRNA genes). The results obtained showed that degradation of n-alkane hydrocarbons was similar between both treatments. However, aromatic compound degradation was more efficient in bioaugmentation treatment, with biodegradation percentages reaching up to 99% in 30 days. Community dynamics was different between treatments and the consortium used in the bioaugmentation treatment contributed to a significant increase in aromatic hydrocarbon degradation. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/52247-1 - Avaliacao do potencial de biossurfactantes de microbiota originada de reservatorios de petroleo para biorremediacao.
• Beneficiário: Bruna Martins Dellagnezze
• Modalidade de apoio: Bolsas no Brasil - Doutorado