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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Prevalence of human papillomavirus types and variants and p16(INK4a) expression in head and neck squamous cells carcinomas in Sao Paulo, Brazil

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Autor(es):
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Betiol, Julio C. [1, 2] ; Sichero, Laura [1, 2, 3] ; de Olival Costa, Henrique O. [4] ; de Matos, Leandro L. [5] ; Andreoli, Maria A. [6] ; Ferreira, Silvaneide [1, 2] ; Faraj, Sheila F. [7] ; de Mello, Evandro S. [7] ; Sobrinho, Joao S. [1, 2] ; Brandao, Lenine G. [5] ; Cernea, Claudio R. [5] ; Kulcsar, Marco A. [5] ; Pinto, Fabio R. [5] ; Goncalves, Antonio J. [8] ; Menezes, Marcelo B. [8] ; Silva, Leonardo [4] ; Rossi, Lia M. [4] ; Lima Nunes, Rafaella A. [6] ; Termini, Lara [6] ; Villa, Luisa L. [9, 1, 2, 6]
Número total de Autores: 20
Afiliação do(s) autor(es):
[1] ICESP, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med, Mol Biol Lab, Hosp Clin, Ctr Translat Res Oncol, Inst Canc Estado Sao Paulo, Sao Paulo - Brazil
[3] Ctr Translat Oncol ICESP, Av Dr Arnaldo 251, 8 Andar, BR-01246000 Sao Paulo, SP - Brazil
[4] Sch Med FCMSCSP, Dept Otolaringol, Santa Casa Sao Paulo, Sao Paulo - Brazil
[5] Univ Sao Paulo, Sch Med, Dept Head & Neck Surg, Sao Paulo - Brazil
[6] Sch Med FCMSCSP, HPV Inst, Santa Casa Sao Paulo, Sao Paulo - Brazil
[7] Canc Inst Sao Paulo ICESP, Dept Pathol, Sao Paulo - Brazil
[8] Sch Med FCMSCSP, Dept Surg, Santa Casa Sao Paulo, Sao Paulo - Brazil
[9] Univ Sao Paulo, Sch Med, Dept Radiol & Oncol, Sao Paulo - Brazil
Número total de Afiliações: 9
Tipo de documento: Artigo Científico
Fonte: INFECTIOUS AGENTS AND CANCER; v. 11, MAY 4 2016.
Citações Web of Science: 5
Resumo

Background: Human papillomavirus (HPV) prevalence in head and neck squamous cell carcinomas (HNSCC) diverges geographically. The reliability of using p16(INK4a) expression as a marker of viral infection is controversial in HNSCC. We evaluated HPV types and HPV-16 variants prevalence, and p16(INK4a) expression in HNSCC specimens provided by two different Institutions in Sao Paulo. Methods: HPV DNA from formalin-fixed specimens was accessed by Inno-LiPA, HPV-16 variants by PCR-sequencing, and p16(INK4a) protein levels by immunohistochemistry. Results: Overall, HPV DNA was detected among 19.4 % of the specimens (36/186). Viral prevalence was higher in the oral cavity (25.0 %, 23/92) then in other anatomical sites (oropharynx 14,3 %, larynx 13.7 %) when samples from both Institutions were analyzed together. HPV prevalence was also higher in the oral cavity when samples from both Institutions were analyzed separately. HPV-16 was the most prevalent type identified in 69.5 % of the HPV positive smaples and specimens were assigned into Asian-American (57.2 %) or European (42.8 %) phylogenetic branches. High expression of p16(INK4a) was more common among HPV positive tumors. Conclusion: Our results support a role for HPV-16 in a subset of HNSCC. (AU)

Processo FAPESP: 08/57889-1 - Instituto de Ciência e Tecnologia para o Estudo das Doenças Associadas ao Papilomavírus
Beneficiário:Luisa Lina Villa
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 11/09616-9 - Diversidade de HPV e risco de câncer
Beneficiário:Luisa Lina Villa
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/01513-9 - Diversidade dè HPV e risco dè câncer
Beneficiário:Julio Cesar Betiol
Linha de fomento: Bolsas no Brasil - Mestrado