Autor(es)

da Costa, C. F. P. ^{[1, 2]} ; Gobbo, M. G. ^{[1, 2]} ; Taboga, S. R. ^{[1, 2]} ; Pinto-Fochi, M. E. ^[2] ; Goes, R. M. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Estadual Paulista IBILCE UNESP, Inst Biosci Letters & Exact Sci, Dept Biol, Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANDROLOGY; v. 4, n. 3, p. 526-541, MAY 2016.
• Citações Web of Science: 6

Resumo

Melatonin may be used as an antioxidant in therapy against systemic sequelae caused by oxidative stress in diabetes. However, as melatonin has a major role in regulating reproductive activity, its consequence on reproductive parameters under diabetes needs to be better clarified. We have studied whether prior and concomitant treatment of juvenile Wistar rats with low doses of melatonin interferes in reproductive damage induced by experimental diabetes after 1 and 8weeks. The consequences of melatonin administration since weaning on reproductive parameters of healthy rats at adulthood were also evaluated. Melatonin was provided in drinking water (10g/kg b.w./day) after weaning (5-week-old). Diabetes was induced by streptozotocin injection (4.5mg/100g b.w.) at 13-week-old rats, and rats were euthanized 1 and 8weeks after disease onset. Diabetes decreased circulating testosterone levels (similar to 35% to 1week; similar to 62% to 2months; p<0.01) but did not affect testes sperm counts. Twomonths of diabetes reduced the sperm reserve and led to atrophy of epididymal cauda. Both 1-week and 2-month diabetes impaired sperm motility, decreased the number of spermatozoa with progressive movement, and increased the number of immotile sperm. Melatonin intake reduced serum testosterone levels similar to 29% in healthy 14-week-old and similar to 23% in 21-week-old rats and reduced daily testicular sperm production similar to 26% in the latter disease stage, but did not interfere in sperm reserves and transit time for both experimental periods. Exogenous melatonin prevented the serum testosterone decrease and damage to sperm motility in diabetic rats and attenuated reduction in sperm counts and transit time induced by 1-week diabetes but did not avoid this decrease at 2-month diabetes. Low doses of melatonin administered prior to and during experimental diabetes attenuated damage to testicular steroidogenic activity and preserved sperm motility, but not sperm reserves in the rat. Our data indicated a differential action of melatonin in normoglycemic and hyperglycemic conditions, particularly in sperm motility and testosterone production by Leydig cells. (AU)

• Processo FAPESP: 10/20756-4 - O papel da melatonina na atenuação dos danos testiculares causados pelo Diabetes experimental
• Beneficiário: Carolina Frandsen Pereira da Costa
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/19467-0 - Administração de melatonina durante a maturação sexual: influência na histofisiologia da próstata adulta e papel protetor contra os danos causados pelo diabetes experimental
• Beneficiário: Marina Guimarães Gobbo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/16071-9 - Efeitos da obesidade materna e supernutrição pós-natal sobre o aparelho genital masculino de ratos: avaliação morfofuncional das células de leydig
• Beneficiário: Maria Etelvina Pinto Fochi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/01612-4 - Efeitos da obesidade materna e supernutrição pós-natal sobre o aparelho genital masculino de ratos: avaliação morfofuncional das células de Leydig
• Beneficiário: Rejane Maira Góes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07210-0 - Baixas doses de melatonina exógena no diabetes experimental: implicações para a estrutura, função e defesa anti-oxidante do testículo e epidídimo de ratos
• Beneficiário: Carolina Frandsen Pereira da Costa
• Modalidade de apoio: Bolsas no Brasil - Mestrado