A capillary electrophoresis system with dual capacitively coupled contactless conductivity detection and electrospray ionization tandem mass spectrometry

Autor(es)

Mendonca Francisco, Kelliton Jose ^[1] ; do Lago, Claudimir Lucio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ELECTROPHORESIS; v. 37, n. 12, p. 1718-1724, JUL 2016.
• Citações Web of Science: 10

Resumo

A commercial system that is comprised of a CE coupled to an ESI triple quadrupole mass spectrometer was equipped with two capacitively coupled contactless conductivity detectors (C(4)Ds). The first (CD)-D-4 was positioned inside the original cartridge, and the second (CD)-D-4 was positioned as close as possible to the ESI probe entrance by using a 3D-printed support. The C(4)Ds electropherograms werematched to the ESI-MS electropherogram by correcting their timescales by the factor L-T/L-D, where L-T and L-D are the total capillary length and the length until the (CD)-D-4, respectively. A general approach formethod development supporting the simultaneous conductivity and MS detection is discussed, while application examples are introduced. These examples include the use of (CD)-D-4 as a simple device that dismiss the use of an EOF marker, a low-selectivity detector that continuously provide information about unexpected features of the sample, and even a detector that can be more sensitive than ESI-MS. The (CD)-D-4 used in this setup proved to have a smaller contribution to the peak broadening than ESI-MS, which allowed that a (CD)-D-4, positioned at 12 cm from the inlet of an 80-cm-long capillary, could be used to foresee position and shape of the peaks being formed 6.8 times slower at the ESI-MS electropherogram. (AU)

• Processo FAPESP: 12/06642-1 - Formação e propriedades de adutos de hidróxi-compostos orgânicos com carbonato em meio aquoso e condições biológicas
• Beneficiário: Claudimir Lucio Do Lago
• Modalidade de apoio: Auxílio à Pesquisa - Regular