Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A capillary electrophoresis system with dual capacitively coupled contactless conductivity detection and electrospray ionization tandem mass spectrometry

Full text
Author(s):
Mendonca Francisco, Kelliton Jose [1] ; do Lago, Claudimir Lucio [1]
Total Authors: 2
Affiliation:
[1] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Sao Paulo, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: ELECTROPHORESIS; v. 37, n. 12, p. 1718-1724, JUL 2016.
Web of Science Citations: 10
Abstract

A commercial system that is comprised of a CE coupled to an ESI triple quadrupole mass spectrometer was equipped with two capacitively coupled contactless conductivity detectors (C(4)Ds). The first (CD)-D-4 was positioned inside the original cartridge, and the second (CD)-D-4 was positioned as close as possible to the ESI probe entrance by using a 3D-printed support. The C(4)Ds electropherograms werematched to the ESI-MS electropherogram by correcting their timescales by the factor L-T/L-D, where L-T and L-D are the total capillary length and the length until the (CD)-D-4, respectively. A general approach formethod development supporting the simultaneous conductivity and MS detection is discussed, while application examples are introduced. These examples include the use of (CD)-D-4 as a simple device that dismiss the use of an EOF marker, a low-selectivity detector that continuously provide information about unexpected features of the sample, and even a detector that can be more sensitive than ESI-MS. The (CD)-D-4 used in this setup proved to have a smaller contribution to the peak broadening than ESI-MS, which allowed that a (CD)-D-4, positioned at 12 cm from the inlet of an 80-cm-long capillary, could be used to foresee position and shape of the peaks being formed 6.8 times slower at the ESI-MS electropherogram. (AU)

FAPESP's process: 12/06642-1 - Formation and properties of adducts of organic hydroxy-compounds with carbonate in aqueous medium and biological conditions
Grantee:Claudimir Lucio Do Lago
Support Opportunities: Regular Research Grants