Exploring C4D detection for the development of innovative and low-cost microfluidic biosensors

• Grant number: 18/19750-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: March 01, 2019
• End date: January 24, 2022
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Emanuel Carrilho
• Grantee: Laís Canniatti Brazaca
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis, AP.TEM

Abstract

Total analysis microsystems (uTAS) are a trend of Modern Chemistry due to the extremely low sample consumption (pL to nL), its portability potential and the integration of several processes into a single device. However, with the miniaturization of analytical systems, several challenges have also arisen, with the main ones being adequate fluid manipulation and the development of detection methods to be used for an accurate and simple analysis. Currently, electrochemical techniques are the commonly applied in uTAS due to its high sensitivity and miniaturization potential. In particular, capacitively coupled contactless conductivity detection (C4D) has been quickly growing in the literature because it does not require electrodes to be in direct contact with the solution, avoiding a number of problems such as electrolysis and metal degradation. However, it is, in principle, a universal detection technique, capable of detecting signals related to any compound with conductivity different from the electrolyte used. Therefore, it has been commonly used along with separation methods, being mainly coupled to capillary electrophoresis (CE-C4D). We propose here the improvement of a new class of innovative biosensors based on the detection of biomolecules selectively and accurately using the C4D technique (uBIA-C4D). For this end, microfluidic devices will be constructed with three gold electrodes: one responsible for sample excitation (eexc), one to act as reference, measuring the basal conductivity of the solution (err), and another to act as a working electrode (erw), evaluating the interactions between the biomolecules. The electrodes will be isolated from the solution by deposition of a SiO2 layer, which will be modified with specific antibodies only on the erw region. It is expected that the difference between the signal obtained by erw and err will vary linearly with the analyte concentration. Previous work by our group shows that the project has great potential to generate successful results. Therefore, we hope to improve this new strategy based on C4D detection for the development of precise, simple, and low cost biosensors to be applied in uTAS.

Scientific publications

(15)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOARES, JULIANA COATRINI; SOARES, ANDREY COATRINI; RODRIGUES, VALQUIRIA CRUZ; OITICICA, PEDRO RAMON ALMEIDA; RAYMUNDO-PEREIRA, PAULO AUGUSTO; BOTT-NETO, JOSE LUIZ; BUSCAGLIA, LORENZO A.; DE CASTRO, LUCAS DANIEL CHIBA; RIBAS, LUCAS C.; SCABINI, LEONARDO; et al. Detection of a SARS-CoV-2 sequence with genosensors using data analysis based on information visualization and machine learning techniques. MATERIALS CHEMISTRY FRONTIERS, JUN 2021. (18/22214-6, 18/19750-3, 18/18953-8, 16/01919-6, 19/13514-9, 14/50867-3, 20/02938-0, 19/00101-8, 19/07811-0, 16/23763-8)

RAFANHIN SOUSA, ANA CAROLINA; MAKARA, CLEYTON NASCIMENTO; BRAZACA, LAIS CANNIATTI; CARRILHO, EMANUEL. A colorimetric microfluidic paper-based analytical device for sulfonamides in cow milk using enzymatic inhibition. Food Chemistry, v. 356, SEP 15 2021. (18/19750-3, 17/19413-4)

TAKEKAWA, VICTOR SADANORY; MARQUES, LETICIA APARECIDA; STRUBINGER, ETHAN; SEGATO, THIAGO PINOTTI; BOGUSZ, JR., STANISLAU; BRAZACA, LAIS CANNIATTI; CARRILHO, EMANUEL. Development of low-cost planar electrodes and microfluidic channels for applications in capacitively coupled contactless conductivity detection ((CD)-D-4). ELECTROPHORESIS, v. 42, n. 16, p. 1560-1569, AUG 2021. (17/19478-9, 18/19750-3)

SILVA, LUIZ R. G.; STEFANO, JESSICA S.; ORZARI, LUIZ O.; BRAZACA, LAIS C.; CARRILHO, EMANUEL; MARCOLINO-JUNIOR, LUIZ H.; BERGAMINI, MARCIO F.; MUNOZ, RODRIGO A. A.; JANEGITZ, BRUNO C.. Electrochemical Biosensor for SARS-CoV-2 cDNA Detection Using AuPs-Modified 3D-Printed Graphene Electrodes. BIOSENSORS-BASEL, v. 12, n. 8, p. 20-pg., 2022-08-01. (17/21097-3, 22/06145-0, 18/19750-3)

BRAZACA, LAIS CANNIATTI; DOS SANTOS, PAMYLA LAYENE; DE OLIVEIRA, PAULO ROBERTO; ROCHA, DIEGO PESSOA; STEFANO, JESSICA SANTOS; KALINKE, CRISTIANE; MUNOZ, RODRIGO ALEJANDRO ABARZA; BONACIN, JULIANO ALVES; JANEGITZ, BRUNO CAMPOS; CARRILHO, EMANUEL. Biosensing strategies for the electrochemical detection of viruses and viral diseases e A review. Analytica Chimica Acta, v. 1159, MAY 15 2021. (18/19750-3, 19/00473-2, 13/22127-2, 19/01844-4, 17/21097-3)

BLASQUES, RODRIGO VIEIRA; STEFANO, JESSICA S.; CAMARGO, JESSICA R.; SLIVA, LUIZ R. GUTERRES E.; BRAZACA, LAIS CANNIATTI; JANEGITZ, BRUNO CAMPOS. Disposable Prussian blue-anchored electrochemical sensor for enzymatic and non-enzymatic multi-analyte detection. SENSORS AND ACTUATORS REPORTS, v. 4, p. 11-pg., 2022-09-01. (18/19750-3, 17/21097-3, 19/23177-0)

BLASQUES, RODRIGO VIEIRA; DE OLIVEIRA, PAULO ROBERTO; KALINKE, CRISTIANE; BRAZACA, LAIS CANNIATTI; CRAPNELL, ROBERT D. D.; BONACIN, JULIANO ALVES; BANKS, CRAIG E. E.; JANEGITZ, BRUNO CAMPOS. Flexible Label-Free Platinum and Bio-PET-Based Immunosensor for the Detection of SARS-CoV-2. BIOSENSORS-BASEL, v. 13, n. 2, p. 19-pg., 2023-02-01. (19/00473-2, 17/21097-3, 18/19750-3, 19/23177-0, 21/07989-4, 13/22127-2)

TAKEKAWA, VICTOR SADANORY; MARQUES, LETICIA APARECIDA; STRUBINGER, ETHAN; SEGATO, THIAGO PINOTTI; BOGUSZ, JR., STANISLAU; BRAZACA, LAIS CANNIATTI; CARRILHO, EMANUEL. Development of low-cost planar electrodes and microfluidic channels for applications in capacitively coupled contactless conductivity detection ((CD)-D-4). ELECTROPHORESIS, JUN 2021. (17/19478-9, 18/19750-3)

SOARES, JULIANA COATRINI; SOARES, ANDREY COATRINI; RODRIGUES, VALQUIRIA CRUZ; OITICICA, PEDRO RAMON ALMEIDA; RAYMUNDO-PEREIRA, PAULO AUGUSTO; BOTT-NETO, JOSE LUIZ; BUSCAGLIA, LORENZO A.; DE CASTRO, LUCAS DANIEL CHIBA; RIBAS, LUCAS C.; SCABINI, LEONARDO; et al. Detection of a SARS-CoV-2 sequence with genosensors using data analysis based on information visualization and machine learning techniques. MATERIALS CHEMISTRY FRONTIERS, v. 5, n. 15, p. 5658-5670, AUG 7 2021. (19/07811-0, 18/19750-3, 20/02938-0, 16/01919-6, 18/18953-8, 19/13514-9, 14/50867-3, 16/23763-8, 18/22214-6, 19/00101-8)

STEFANO, JESSICA SANTOS; GUTERRES E SILVA, LUIZ RICARDO; ROCHA, RAQUEL GOMES; BRAZACA, LAIS CANNIATTI; RICHTER, EDUARDO MATHIAS; ABARZA MUNOZ, RODRIGO ALEJANDRO; JANEGITZ, BRUNO CAMPOS. New conductive filament ready-to-use for 3D-printing electrochemical (bio)sensors: Towards the detection of SARS-CoV-2. Analytica Chimica Acta, v. 1191, JAN 25 2022. (17/21097-3, 18/19750-3)

SILVA, LUIZ RICARDO GUTERRES; STEFANO, JESSICA SANTOS; NOCELLI, ROBERTA CORNELIO FERREIRA; JANEGITZ, BRUNO CAMPOS. 3D electrochemical device obtained by additive manufacturing for sequential determination of paraquat and carbendazim in food samples. Food Chemistry, v. 406, p. 9-pg., 2023-04-16. (17/21097-3, 22/06145-0, 18/19750-3)

MAURUTO DE OLIVEIRA, GABRIELA C.; DE SOUZA CARVALHO, JEFFERSON HENRIQUE; BRAZACA, LAIS CANNIATTI; SILVA VIEIRA, NIRTON CRISTI; JANEGITZ, BRUNO CAMPOS. Flexible platinum electrodes as electrochemical sensor and immunosensor for Parkinson's disease biomarkers. BIOSENSORS & BIOELECTRONICS, v. 152, MAR 15 2020. (17/17572-8, 18/07508-3, 19/04085-7, 17/21097-3, 18/19750-3, 17/21898-6)

BRAZACA, LAIS CANNIATTI; SAMPAIO, ISABELLA; ZUCOLOTTO, VALTENCIR; JANEGITZ, BRUNO CAMPOS. Applications of biosensors in Alzheimer's disease diagnosis. Talanta, v. 210, APR 1 2020. (17/21898-6, 17/21097-3, 15/02623-0, 18/19750-3)

BRAZACA, LAIS CANNIATTI; IMAMURA, AMANDA HIKARI; GOMES, NATHALIA OEZAU; ALMEIDA, MARIANA BORTHOLAZZI; SCHEIDT, DESIREE TAMARA; RAYMUNDO-PEREIRA, PAULO A.; OLIVEIRA JR, OSVALDO N.; JANEGITZ, BRUNO CAMPOS; SPINOLA MACHADO, SERGIO ANTONIO; CARRILHO, EMANUEL. Electrochemical immunosensors using electrodeposited gold nanostructures for detecting the S proteins from SARS-CoV and SARS-CoV-2. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 414, n. 18, p. 11-pg., 2022-02-15. (20/09587-8, 17/21097-3, 19/01777-5, 17/05362-9, 18/22214-6, 16/01919-6, 18/19750-3)

FEITOR, JESSICA F.; BRAZACA, LAIS C.; LIMA, AMANDA M.; FERREIRA, VINICIUS G.; KASSAB, GIULIA; BAGNATO, VANDERLEI S.; CARRILHO, EMANUEL; CARDOSO, DANIEL R.. Organ-on-a-Chip for Drug Screening: A Bright Future for Sustainability? A Critical Review. ACS BIOMATERIALS SCIENCE & ENGINEERING, v. 9, n. 5, p. 15-pg., 2023-04-04. (17/01189-0, 18/19750-3, 18/18188-0)