Polymeric Nanoparticle-Based Photodynamic Therapy for Chronic Periodontitis in Vivo

Autor(es)

de Freitas, Laura Marise ^[1] ; Fioramonti Calixto, Giovana Maria ^[2] ; Chorilli, Marlus ^[2] ; Giusti, Jucaira Stella M. ^[3] ; Bagnato, Vanderlei Salvador ^[3] ; Soukos, Nikolaos S. ^[4] ; Amiji, Mansoor M. ^[5] ; Fontana, Carla Raquel ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Anal Clin, Campus Araraquara, BR-14800903 Araraquara, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Farmacos & Medicamentos, Campus Araraquara, BR-14800903 Araraquara, SP - Brazil

^[3] Univ Sao Paulo, Inst Fis Sao Carlos, Caixa Postal 369, BR-15980900 Sao Carlos, SP - Brazil

^[4] Forsyth Inst, Appl Mol Photomed Lab, 245 First St, Cambridge, MA 02142 - USA

^[5] Northeastern Univ, Sch Pharm, Dept PharmaceuticalSci, Bouve Coll Hlth Sci, 140 Fenway, Room 156, 360 Huntington Ave, Boston, MA 02115 - USA

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 17, n. 5 MAY 2016.
• Citações Web of Science: 16

Resumo

Antimicrobial photodynamic therapy (aPDT) is increasingly being explored for treatment of periodontitis. Here, we investigated the effect of aPDT on human dental plaque bacteria in suspensions and biofilms in vitro using methylene blue (MB)-loaded poly(lactic-co-glycolic) (PLGA) nanoparticles (MB-NP) and red light at 660 nm. The effect of MB-NP-based aPDT was also evaluated in a clinical pilot study with 10 adult human subjects with chronic periodontitis. Dental plaque samples from human subjects were exposed to aPDT-in planktonic and biofilm phases-with MB or MB-NP (25 mu g/mL) at 20 J/cm(2) in vitro. Patients were treated either with ultrasonic scaling and scaling and root planing (US + SRP) or ultrasonic scaling + SRP + aPDT with MB-NP (25 mu g/mL and 20 J/cm(2)) in a split-mouth design. In biofilms, MB-NP eliminated approximately 25% more bacteria than free MB. The clinical study demonstrated the safety of aPDT. Both groups showed similar improvements of clinical parameters one month following treatments. However, at three months ultrasonic SRP + aPDT showed a greater effect (28.82%) on gingival bleeding index (GBI) compared to ultrasonic SRP. The utilization of PLGA nanoparticles encapsulated with MB may be a promising adjunct in antimicrobial periodontal treatment. (AU)

• Processo FAPESP: 12/21203-4 - Análise microbiológica do efeito da terapia fotodinâmica em infecções bacterianas
• Beneficiário: Carla Raquel Fontana Mendonça
• Linha de fomento: Auxílio à Pesquisa - Regular