A new heterologous fibrin sealant as a scaffold to cartilage repair-Experimental study and preliminary results

Autor(es)

de Barros, Caio Nunes ^[1] ; Miluzzi Yamada, Ana Lucia ^[1] ; Junior, Rui Seabra F. ^[2] ; Barraviera, Benedito ^[2] ; Hussni, Carlos Alberto ^[1] ; de Souza, Jaqueline Brandao ^[1] ; Watanabe, Marcos Jun ^[1] ; Rodrigues, Celso Antonio ^[1] ; Garcia Alves, Ana Liz ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP Univ Estadual Paulista, Sch Vet Med & Anim Sci, Dept Vet Surg & Anesthesiol, BR-18618970 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, UNESP Univ Estadual Paulista, Ctr Study Venoms & Venomous Anim, BR-18618970 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental Biology and Medicine; v. 241, n. 13, p. 1410-1415, JUL 2016.
• Citações Web of Science: 5

Resumo

Autologous fibrin gel is commonly used as a scaffold for filling defects in articular cartilage. This biomaterial can also be used as a sealant to control small hemorrhages and is especially helpful in situations where tissue reparation capacity is limited. In particular, fibrin can act as a scaffold for various cell types because it can accommodate cell migration, differentiation, and proliferation. Despite knowledge of the advantages of this biomaterial and mastery of the techniques required for its application, the durability of several types of sealant at the site of injury remains questionable. Due to the importance of such data for evaluating the quality and efficiency of fibrin gel formulations on its use as a scaffold, this study sought to analyze the heterologous fibrin sealant developed from the venom of Crotalus durissus terrificus using studies in ovine experimental models. The fibrin gel developed from the venom of this snake was shown to act as a safe, stable, and durable scaffold for up to seven days, without causing adverse side effects. Fibrin gel produced from the venom of the Crotalus durissus terrificus snake possesses many clinical and surgical uses. It presents the potential to be used as a biomaterial to help repair skin lesions or control bleeding, and it may also be used as a scaffold when applied together with various cell types. The intralesional use of the fibrin gel from the venom of this snake may improve surgical and clinical treatments in addition to being inexpensive and adequately consistent, durable, and stable. The new heterologous fibrin sealant is a scaffold candidate to cartilage repair in this study. (AU)

• Processo FAPESP: 12/04516-9 - Gel de plaquetas em defeitos da cartilagem articular: estudo in vitro e in vivo em ovinos
• Beneficiário: Caio Nunes de Barros
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/03600-6 - Células tronco mesenquimais e plasma rico em plaquetas associados com microfraturas no tratamento de lesões condrais articulares em equinos
• Beneficiário: Ana Liz Garcia Alves
• Modalidade de apoio: Auxílio à Pesquisa - Regular