Development of Molecularly Imprinted Polymers for Solid Phase Extraction of Parabens in Plasma Samples and Analysis by UHPLC-MS/MS

Autor(es)

Mariane V. Roldão ^[1] ; Lidervan P. Melo ^[2] ; Luis Felippe C. Miranda ^[3] ; Marcos G. Resende ^[4] ; Maria Eugênia C. Queiroz ^[5]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Faculdade de Filosofia Ciência e Letras de Ribeirão Preto. Departamento de Química - Brasil

^[2] Universidade de São Paulo. Faculdade de Filosofia Ciência e Letras de Ribeirão Preto. Departamento de Química - Brasil

^[3] Universidade de São Paulo. Faculdade de Filosofia Ciência e Letras de Ribeirão Preto. Departamento de Química - Brasil

^[4] Universidade de São Paulo. Faculdade de Filosofia Ciência e Letras de Ribeirão Preto. Departamento de Química - Brasil

^[5] Universidade de São Paulo. Faculdade de Filosofia Ciência e Letras de Ribeirão Preto. Departamento de Química - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 28, n. 2, p. 257-265, 2017-02-00.

Resumo

Two molecularly imprinted polymers (MIPs) were synthesized, by sol-gel and radical polymerization procedures. These MIPs were evaluated as selective stationary phases for miniaturized solid phase extraction (MISPE) to determine parabens in plasma samples by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Selective cavities of the MIPs, based on molecular recognition (hydrogen bonds), were revealed after removal of the template (benzylparaben). Both MIPs were characterized by scanning electron microscope (SEM) and Fourier-transform infrared spectroscopy (FTIR). MIP prepared by radical process presented higher selective binding capacity for the target parabens than MIP prepared by sol-gel. Several variables that influence MISPE performance, including sample pH, sorbent washing, elution solvent, and elution solvent volume were also evaluated. According to the analytical validation parameters, the MISPE/UHPLC-MS/MS method is suitable for the analysis of parabens in plasma samples. The effectiveness of this method was demonstrated through the analyses of the umbilical plasma samples from postpartum women. (AU)

• Processo FAPESP: 12/21178-0 - Desenvolvimento da fase extratora molecularmente impressa para análises de parabenos em amostras de plasma por cromatografia líquida acoplada a espectrometria de massas sequencial
• Beneficiário: Mariane Valéria Roldão
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica