Salivary Biomarkers for Detection of Oral Squamous Cell Carcinoma in a Taiwanese Population

Autor(es)

Gleber-Netto, Frederico Omar ; Yakob, Maha ; Li, Feng ; Feng, Ziding ; Dai, Jianliang ; Kao, Huang-Kai ; Chang, Yu-Liang ; Chang, Kai-Ping ; Wong, David T. W.

• Tipo de documento: Artigo Científico
• Fonte: Clinical Cancer Research; v. 22, n. 13, p. 3340-3347, JUL 1 2016.
• Citações Web of Science: 12

Resumo

Purpose: This study evaluated the discriminatory power of salivary transcriptomic and proteomic biomarkers in distinguishing oral squamous cell carcinoma cases from controls and potentially malignant oral disorders (PMOD). Experimental Design: A total of 180 samples (60 OSCC patients, 60 controls, and 60 PMOD patients) were used in the study. Seven transcriptomic markers (IL8, IL1 beta, SAT1, OAZ1, DUSP1, S100P, and H3F3A) were measured using qPCR, and two proteomic markers (IL8 and IL1 beta) were evaluated by ELISA. Results: Among 7 transcriptomic markers, transcript level of DUSP1 was significantly lower in OSCC patients than in controls and PMOD patients. Between the proteomic markers, the protein concentration of IL8 and IL1 beta was significantly higher in OSCC patients than controls and dysplasia patients. Univariate fractional polynomial (FP) models revealed that salivary IL8 protein (IL8p) has the highest AUC value between OSCC patients and controls (0.74) and between OSCC and PMOD patients (0.72). Applying a 2-marker FPmodel, salivary IL8p combined with IL1 beta gave the best AUC value for discrimination between OSCC patients and controls, as well as the IL8p combined with H3F3AmRNA, which gave the best AUC value for discrimination between OSCC and PMOD patients. Multivariate models analysis combining salivary analytes and risk factor exposure related to oral carcinogenesis formed the best combinatory variables for differentiation between OSCC versus PMOL (AUC = 0.80), OSCC versus controls (AUC = 0.87), and PMOD versus controls (AUC = 0.78). Conclusions: The combination of transcriptomic and proteomic salivary markers is of great value for oral cancer detection and differentiation from PMOD patients and controls. (C) 2016 AACR. (AU)

• Processo FAPESP: 11/13315-4 - Estudo em larga-escala de alterações transcricionais relacionadas à ocorrência de metástases no câncer oral.
• Beneficiário: Frederico Omar Gleber Netto
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/09142-2 - Estudo de marcadores salivares relacionados à metástase nodal em carcinoma epidermóide de boca
• Beneficiário: Frederico Omar Gleber Netto
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado