HLA-DQA1/B1 alleles as putative susceptibility markers in congenital toxoplasmosis

Autor(es)

Shimokawa, Paulo Tadashi ; Targa, Lilia Spaleta ; Yamamoto, Lidia ; Rodrigues, Jonatas Cristian ; Kanunfre, Kelly Aparecida ; Okay, Thelma Suely

• Tipo de documento: Artigo Científico
• Fonte: VIRULENCE; v. 7, n. 4, p. 456-464, 2016.
• Citações Web of Science: 2

Resumo

Host and parasite genotypes are among the factors associated with congenital toxoplasmosis pathogenesis. As HLA class II molecules play a key role in the immune system regulation, the aim of this study was to investigate whether HLA-DQA1/B1 alleles are associated with susceptibility or protection to congenital toxoplasmosis. One hundred and twenty-two fetuses with and 103 without toxoplasmosis were studied. The two study groups were comparable according to a number of socio-demographic and genetic variables. HLA alleles were typed by PCR-SSP. In the HLA-DQA1 region, the allele frequencies showed that {*}01:03 and {*}03:02 alleles could confer susceptibility ( OR= 3.06, p = 0.0002 and OR=9.60, p= 0.0001, respectively) as they were more frequent among infected fetuses. Regarding the HLA-DQB1 region, the {*}05: 04 allele could confer susceptibility ( OR = 6.95, p < 0.0001). Of the 122 infected fetuses, 10 presented susceptibility haplotypes contrasting with only one in the non-infected group. This difference was not statistically significant after correction for multiple comparison ( OR = 9.37, p=0.011). In the casuistic, there were two severely damaged fetuses with high parasite loads determined in amniotic fluid samples and HLA-DQA1 susceptibility alleles. In the present study, a discriminatory potential of HLA-DQA1/B1 alleles to identify susceptibility to congenital toxoplasmosis and the most severe cases has been shown. (AU)

• Processo FAPESP: 10/15022-1 - Frequência de alelos do HLA-DQA1 e DQB1 em fetos com toxoplasmose: associação com os genótipos parasitários e a carga parasitária
• Beneficiário: Thelma Suely Okay
• Modalidade de apoio: Auxílio à Pesquisa - Regular