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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Bioavailability of catechins from guarana (Paullinia cupana) and its effect on antioxidant enzymes and other oxidative stress markers in healthy human subjects

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Yonekura, Lina ; Martins, Carolina Aguiar ; Sampaio, Geni Rodrigues ; Monteiro, Marcela Piedade ; Machado Cesar, Luiz Antonio ; Mioto, Bruno Mahler ; Mori, Clara Satsuki ; Nogueira Mendes, Thaise Maria ; Ribeiro, Marcelo Lima ; Arcari, Demetrius Paiva ; Ferraz da Silva Torres, Elizabeth Aparecida
Número total de Autores: 11
Tipo de documento: Artigo Científico
Fonte: FOOD & FUNCTION; v. 7, n. 7, p. 2970-2978, 2016.
Citações Web of Science: 18
Resumo

We assessed the effects of guarana (Paullinia cupana) consumption on plasma catechins, erythrocyte antioxidant enzyme activity (superoxide dismutase, catalase and glutathione peroxidase) and biomarkers of oxidative stress (ex vivo LDL oxidation, plasma total antioxidant status and ORAC, and lymphocyte single cell gel electrophoresis) in healthy overweight subjects. Twelve participants completed a 15-day run-in period followed by a 15-day intervention with a daily intake of 3 g guarana seed powder containing 90 mg (+)-catechin and 60 mg (-)-epicatechin. Blood samples were taken on the first and last day of the intervention period, fasting and 1 h post-dose. The administration of guarana increased plasma ORAC, while reducing ex vivo LDL oxidation (only in the first study day) and hydrogen peroxide-induced DNA damage in lymphocytes, at 1 h post-dose. Plasma catechin (0.38 +/- 0.12 and 0.44 +/- 0.18 nmol mL(-1)), epicatechin (0.59 +/- 0.18 and 0.64 +/- 0.25 nmol mL(-1)) and their methylated metabolites were observed at 1 h post-dose but were almost negligible after overnight fasting. The activities of catalase (in both study days) and glutathione peroxidase (in the last intervention day) increased at 1 h post-dose. Furthermore, the activity of both enzymes remained higher than the basal levels in overnight-fasting individuals on the last intervention day, suggesting a prolonged effect of guarana that continues even after plasma catechin clearance. In conclusion, guarana catechins are bioavailable and contribute to reduce the oxidative stress of clinically healthy individuals, by direct antioxidant action of the absorbed phytochemicals and up-regulation of antioxidant/detoxifying enzymes. (AU)

Processo FAPESP: 09/08282-0 - Bioacessibilidade, biodisponibilidade e atividade antioxidante de compostos fenólicos do guaraná (Paullinia cupana) in vitro e in vivo
Beneficiário:Lina Yonekura
Linha de fomento: Bolsas no Brasil - Apoio a Jovens Pesquisadores
Processo FAPESP: 08/10308-4 - Bioacessibilidade, biodisponibilidade e atividade antioxidante de compostos fenólicos do Guaraná (Paullinia cupana) in vitro e in vivo
Beneficiário:Lina Yonekura
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores