The role of oxidative stress in streptozotocin-induced diabetic nephropathy in rats

Autor(es)

Fernandes, Sheila Marques ; Cordeiro, Priscilla Mendes ; Watanabe, Mirian ; da Fonseca, Cassiane Dezoti ; Fernandes Vattimo, Maria de Fatima

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ENDOCRINOLOGY METABOLISM; v. 60, n. 5, p. 443-449, OCT 2016.
• Citações Web of Science: 11

Resumo

Objective: The objective of this study was to evaluate the role of oxidative stress in an experimental model of streptozotocin-induced diabetic nephropathy in rats. Materials and methods: Wistar, adult, male rats were used in the study. Animals were divided in the following groups: Citrate (control, citrate buffer 0.01M, pH 4.2 was administrated intravenously -i.v - in the caudal vein), Uninephrectomy+Citrate (left uninephrectomy-20 days before the study), DM (streptozotocin, 65 mg/kg, i.v, on the 20th day of the study), Uninephrectomy+DM. Physiological parameters (water and food intake, body weight, blood glucose, kidney weight, and relative kidney weight); renal function (creatinine clearance), urine albumin (immunodiffusion method); oxidative metabolites (urinary peroxides, thiobarbituric acid reactive substances, and thiols in renal tissue), and kidney histology were evalua-ted. Results: Polyphagia, polydipsia, hyperglycemia, and reduced body weight were observed in diabetic rats. Renal function was reduced in diabetic groups (creatinine clearance, p < 0.05). Uninephrectomy potentiated urine albumin and increased kidney weight and relative kidney weight in diabetic animals (p < 0.05). Urinary peroxides and thiobarbituric acid reactive substances were increased, and the reduction in thiol levels demonstrated endogenous substrate consumption in diabetic groups (p < 0.05). The histological analysis revealed moderate lesions of diabetic nephropathy. Conclusion: This study confirms lipid peroxidation and intense consumption of the antioxidant defense system in diabetic rats. The association of hyperglycemia and uninephrectomy resulted in additional renal injury, demonstrating that the model is adequate for the study of diabetic nephropathy. (AU)

• Processo FAPESP: 11/24028-6 - O efeito do inibidor da fosfodiestarese-5 na lesão renal aguda isquêmica em ratos
• Beneficiário: Mirian Watanabe
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/26560-2 - O contraste radiológico em situações de risco para nefropatia
• Beneficiário: Maria de Fatima Fernandes Vattimo
• Modalidade de apoio: Auxílio à Pesquisa - Regular