Sperm quality and fertility in rats after prenatal exposure to low doses of TCDD: A three-generation study

Autor(es)

Sanabria, Marciana ; Cucielo, Maira Smaniotto ; Guerra, Marina Trevizan ; Borges, Cibele dos Santos ; Banzato, Thais Petrochelli ; Perobelli, Juliana Elaine ; Araujo Leite, Gabriel Adan ; Anselmo-Franci, Janete Aparecida ; Kempinas, Wilma De Grava

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTIVE TOXICOLOGY; v. 65, p. 29-38, OCT 2016.
• Citações Web of Science: 12

Resumo

Exposure to Tetrachlorodibenzo-p-dioxin (TCDD) in male rats promotes, decreased sperm concentration, alterations in motility and in sperm transit time. We evaluated the effect transgenerational of in utero exposure to low doses TCDD in the sperm quality. Pregnant rats (F0) were exposed to 0.1; 0.5 and 1.0 mu g of TCDD, on gestational day 15, coincides with the end of most organogenesis in the fetus. Adult male offspring (F1, F2 and F3 generation) were investigated for fertility after artificial insemination in utero. After collection of the uterus and ovaries, the numbers of corpora lutea and implants were determined. TCDD provoked alterations in sperm morphology and diminution in serum testosterone levels and sperm transit time in the cauda epididymis. The fertility significantly decreased in all the generations, at least at one dose. In conclusion, TCDD exposure decreases rat sperm quality and fertility in adult male offspring and this effects persist into the next generation. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/13312-8 - Toxicologia reprodutiva de ratos adultos expostos ao 2,3,7,8-tetraclorodibenzo-p-dioxina (TCDD) in utero e durante a lactação
• Beneficiário: Maira Smaniotto Cucielo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/00365-6 - Toxicologia reprodutiva de ratos adultos expostos ao 2,3,7,8-tetraclorodibenzo-p-dioxina (TCDD) in utero e durante a lactação
• Beneficiário: Marciana Sanabria
• Modalidade de apoio: Bolsas no Brasil - Doutorado